1. Specimen Collection, Processing, Culture, and Biochemical Identification of Acinetobacter spp.

Binesh Lal, Saranya Vijayakumar, Shalini Anandan, and Balaji Veeraraghavan

2. Genotyping of Acinetobacter baumannii in Nosocomial Outbreak and Surveillance

Saranya Vijayakumar, Balaji Veeraraghavan, Agila K Pragasam, and Yamuna Devi Bakthavachalam

3. Antimicrobial Susceptibility Testing Methods for Acinetobacter spp

Balaji Veeraraghavan, Saranya Vijayakumar, Agila K Pragasam, Yamuna Devi Bakthavachalam, and John A J Prakash

4. Methods to Evaluate Colistin Heteroresistance in Acinetobacter baumannii

Edgar X. Sherman, Jessie E. Wozniak, and David S. Weiss

5. Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper Supplemented Liquid Media and on Copper-Containing Surfaces

6. A New Method for Determination of Minimum Biofilm Eradication Concentration for Accurate Antimicrobial Therapy

Nithin Sam, Raziya Fathima, and Balaji Veeraraghavan

7. Transformation of Acinetobacter baumannii: Electroporation

Mitchell G. Thompson and Süleyman Yildirim

8. Methods for Natural Transformation in Acinetobacter baumannii

Gottfried Wilharm and Evelyn Skiebe

9. Vesicle Mediated Gene Transfer in A. baumannii

Shweta Fulsundar, Sara Domingues and Kaare M. Nielsen

10. Targeted Gene Replacement in Acinetobacter baumannii

Indranil Biswas and Joshua Mettlach

11. RecET-Mediated Recombineering in Acinetobacter baumannii

Ashley T. Tucker, Matthew J. Powers, M. Stephen Trent, and Bryan W. Davies

Larry A. Gallagher

13. Tn7-Based Single Copy Insertion Vectors for Acinetobacter baumannii

14. Distinguishing Colony Opacity Variants and Measuring Opacity Variation in Acinetobacter baumannii

Sarah E. Anderson and Philip N. Rather

15. A Simple Static Biofilm Assay for Acinetobacter baumannii

Indranil Biswas and Joshua Mettlach

16. BioFlux^TM 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth

Bertrand Naudin, Anja Heins, Stéphane Pinhal, Emmanuelle Dé^, and Marion Nico

17. In Vitro Motility Assays for Acinetobacter Species

Indranil Biswas, Alexandra Machen and Joshua Mettlach

Xun Wang, M. Stephen Trent, and Bryan W. Davies

19. Measuring Intracellular Metal Concentration via ICP-MS Following Copper Exposure

Caitlin L. Williams, Heather M. Neu, Sarah L.J. Michel, and D. Scott Merrell

20. PBP Isolation and DD-Carboxypeptidase Assay

Shilpa Pal and Anindya S. Ghosh

21. Extraction and Visualization of Capsular Polysaccharide from Acinetobacter baumannii

Kyle A. Tipton and Philip N. Rather

22. Isolation of Lipid Cell Envelope Components from Acinetobacter baumannii