Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS
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Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS

Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS


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About the Book

Il metodo presenta una procedura di estrazione efficiente basata sull'estrazione liquido-liquido (LLE) che fornisce un recupero elevato e costante per entrambi gli analiti. L'effetto matrice è stato accuratamente valutato stimando i fattori di matrice normalizzati IS anche attraverso l'infusione dell'analita dopo la colonna. Il metodo LC-MS/MS sviluppato per la quantificazione di ART, DHA, LF e DLF nel plasma umano è stato completamente validato secondo le linee guida della FDA statunitense. Il metodo proposto è sensibile per entrambi gli analiti rispetto a tutti gli altri metodi presentati sia come singolo analita che in combinazione in diverse matrici biologiche. Il metodo offre vantaggi significativi rispetto a quelli precedentemente riportati, in termini di minore necessità di campioni per l'analisi, semplicità della procedura di estrazione e tempo complessivo di analisi. L'assenza di interferenze della matrice è efficacemente dimostrata dall'infusione postcolonna e dai valori di precisione (% CV) per le pendenze calcolate delle curve di calibrazione. Il metodo validato ha mostrato dati accettabili per tutti i parametri di validazione, con sensibilità e selettività adeguate per la loro quantificazione simultanea in ambito clinico.


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Product Details
  • ISBN-13: 9786205530221
  • Publisher: KS Omniscriptum Publishing
  • Binding: Paperback
  • Language: Italian
  • Returnable: N
  • Weight: 209 gr
  • ISBN-10: 6205530228
  • Publisher Date: 20 May 2023
  • Height: 229 mm
  • No of Pages: 100
  • Spine Width: 6 mm
  • Width: 152 mm


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Analisi dei farmaci antimalarici nel plasma umano mediante LC-MS/MS
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