Analytical Biochemistry and Separation Techniques
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Analytical Biochemistry and Separation Techniques

Analytical Biochemistry and Separation Techniques


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About the Book

This laboratory manual is the outcome of teaching in this area for the past 12 years to our M.Sc. Biotechnology students under National Biotechnology Training Program of DBT, New Delhi. This book consists of ~30 Chapters and is organized into 5 Parts. Part- I essentially describes the General Instructions for using the laboratory. Part- II presents in detail the common Biochemical Techniques used at B.Sc. and M.Sc. levels, whereas Part- III, describes with Separation Techniques such as Electrophoretic Techniques (SDS, Agarose, IEF, 2D gels, Pulse-Field Gels, Capillary Electrophoreses, etc.) Basic Chromatographic Techniques (Adsorption, Paper, TLC, IEC, Gel filtration, etc., as well as Advanced Chromatographic Techniques such as Hydroxyapatite, DNA-Cellulose, dHPLC, UPLC, FPLC); MALDI-ToF (Tandem MS). Part- IV deals with Enzymes and Enzyme Techniques such as basic enzyme kinetics, assay of enzymes (Amylases, Alkaline/Acid Phosphatases, Invertases, Proteases, DNA Polymerases, Restriction Endonucleases), Purification of enzymes including restriction endonucleases and zymogram analysis of enzymes. The Appendix section (Part- IV) covers the latest periodic table, properties of commonly used acids and bases, pka & pI values of buffer components and amino acids, codon usage, commonly used bacterial strains and their phenotypes for rDNA experiments, various media compositions for bacteria and fungi including Pichia, molecule weight markers for DNA, RNA and Protein gels, Ammonium sulphate precipitation table, radioactive decay table, tables for preparation of buffers. In each Chapter the background and basic principles of the experiments are explained in sufficient detail with illustrations and along with the experimental protocols. Experimental results (wherever available) obtained under Indian laboratory conditions are given for students’ reference. Useful for conducting practical classes for undergraduate and postgraduate students of Life Sciences.


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Product Details
  • ISBN-13: 9788190848909
  • Publisher: Twentyfirst Century Publications
  • Binding: Textbook
  • ISBN-10: 8190848909
  • Publisher Date: 01 Jan 2000


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