Part I: Developmental Biology

1. The Embryonic Mouse Hindbrain Model to Study Sprouting Angiogenesis In Vivo

Gabriela D'Amico and Christiana Ruhrberg

2. A Workflow to Track and Analyze Endothelial Migration during Vascular Development in Zebrafish Embryos Using Lightsheet Microscopy

Yan Chen, Paul C. Evans, and Robert N. Wilkinson

3. Analyzing Vessel Regression and Endothelial Apoptosis as a Component of Angiogenic Vessel Remodeling

Lachlan Whitehead, Emma C. Watson, and Zoe L. Grant

4. Whole-Mount Immunofluorescence Protocol for 3D Imaging, Reconstruction, and Quantification of 4th Pharyngeal Arch Artery Formation

Elena Ioannou and Christiana Ruhrberg

5. Quantifying and Characterizing Angiogenesis Using the Postnatal Mouse Retina

Alessandro Fantin

Part II: Adult Physiology and Pathology

6. Characterization of Lymphatic Vasculature Using Whole-Mount Immunostaining of Mouse Embryonic Dorsal Skin

Nikita Ved and Jacinta Isabelle Kalisch-Smith

7. Analyzing Lymphatic Vessel Patterning in Adult Tissue

Louise A. Johnson

8. Transmission Electron Microscopy of Endothelium

Zubair Ahmed Nizamundeen, Loubna ElSaboni, Grace Vetch, Jumana Karim, Chris R. Neal, Claire L. Allen, David O. Bates, and Kenton P. Arkill

9. Measurement of Revascularisation in the Hind Limb after Experimental Ischemia in Mice

Sohni Ria Bhalla, Federica Riu, Maria J.C. Machado, and David O. Bates

10. Imaging Blood Vessels and Lymphatics in Mouse Trachea Wholemounts

Peter Baluk and Donald M. McDonald

11. Non-Invasive Measurement of Retinal Microvascular Permeability during Loss of Endothelial Quiescence

Naseeb K. Malhi, David O. Bates, Kenton P. Arkill, and Claire L. Allen

12. An Ex Vivo Tissue Culture Method for Discovering Cell Dynamics Involved in Stromal Vascular Fraction Vasculogenesis Using the Mouse Mesentery

Dima Majbour, Ariana D. Suarez-Martinez, Nicholas A. Hodges, Arinola O. Lampejo, Banks M. Lomel, Elijah W. Rice, Hulan Shang, Adam J. Katz, and Walter L. Murfee

13. Quantitative Three-Dimensional Analysis of the Lymphatic Vasculature in the Postnatal Mouse Heart

Konstantinos Klaourakis, Paul R. Riley, and Joaquim Miguel Vieira

14. Quantifying Lymphatic Vessel Density in Human Tissue Samples

Stewart G. Martin, Emad Rakha, and Sarah J. Storr

15. Quantifying Vascular Remodeling in the Mouse Spinal Cord

M.E. Da Vitoria Lobo, Lydia Hardowar, and Richa