About the Book
Part I: Microscopic Assessment
1. Assessing Tumor Angiogenesis in Histological Samples
Jia-Min Pang, Nicholas Jene, and Stephen B Fox
2. Immunohistochemical Methods for Measuring Tissue Lymphangiogenesis
Daniel J. Royston, Steven Clasper, and David G. Jackson
3. Immunohistochemical Assessment of Leukocyte Involvement in Angiogenesis Narmeen S. Ahmad, Stewart G. Martin, and Sarah J. Storr
Part II: Cell Isolation Techniques
4. Isolation and Culture of Human Endothelial Cells from Micro- And Macro-Vessels
Peter W Hewett
5. Isolation, Identification and Culture of Human Lymphatic Endothelial Cells
Zerina Lokmic
6. Isolation, Culture and Characterisation of Vascular Smooth Muscle Cells
Jessal J Patel and Richard CM Siow
7. Isolation and Transfection of Primary Culture Bovine Retinal Pericytes
Vincent A. Primo and Joseph F. Arboleda-Velasquez
Part III: In Vitro Techniques
8. In Vitro Assays for Endothelial Cell Functions Required for Angiogenesis: Proliferation, Motility, Tubular Differentiation And Matrix Proteolysis
Suzanne A Eccles, William Court, and Lisa Patterson
9. Tube Forming Assays
Ryan M. Brown, Christopher J. Meah, Victoria L. Heath, Iain B. Styles, and Roy Bicknell
10. In Vitro Co-Culture Assays of Angiogenesis
Mark Richards and Harry Mellor
11. Spheroid-Based In Vitro Angiogenesis Model
Larissa Pfisterer and Thomas Korff
12. Stem Cell Spheroid-Based Sprout Assay in Three-Dimensional Fibrin Scaffold: A Novel In Vitro Model for the Study Of Angiogenesis
Fatemeh Sharifpanah and Heinrich Sauer
13. Human Arterial Ring Angiogenesis Assay
Giorgio Seano and Luca Primo
14. A Modified Aortic Ring Assay to Assess Angiogenic Potential In Vitro
Nina Zippel, Yindi Ding, and Ingrid Fleming
15. Quantitative Imaging-Based Examination of Pericyte Control of Endothelial Growth Dynamics and Angiogenesis.
Anthony R. Sheets, Jennifer T. Durham, and Ira M. Herman
16. Static and Dynamic Assays of Cell Adhesion Relevant to the Vasculature
Lynn M. Butler, Helen M. Mcgettrick, and Gerard B. Nash
Part IV: In Vivo Techniques
17. Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy
Axel Sckell and Michael Leunig
18. Quantitative Estimation of Tissue Blood Flow Rate
Gillian M Tozer, Vivien E Prise, and Vincent J Cunningham
19. Chorioallantoic Membrane Microtumor Model to Study the Mechanisms of Tumor Angiogenesis, Vascular Permeability, and Tumor Cell Intravasation
Elena I. Deryugina
20. The Rabbit Corneal Pocket Assay
Lucia Morbidelli and Marina Ziche
21. The Corneal Micropocket Assay: A Model of Angiogenesis and Lymphangiogenesis
Shintaro Nakao and Ali Hafezi-Moghadam
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