Part I: Production and Design
1. Antibiotics - Precious Goods in Changing Times
Peter Sass
2. Mining Bacterial Genomes for Secondary Metabolite Gene Clusters
Martina Adamek, Marius Spohn, Evi Stegmann, and Nadine Ziemert
3. Production of Antimicrobial Compounds by Fermentation
Henrik Harms, Gabriele M. König and Till F. Schäberle
4. Structure Elucidation of Antibiotics by NMR Spectroscopy
Georgios Daletos, Elena Ancheeva, Raha Orfali, Victor Wray, and Peter Proksch
5. Computer-Aided Drug Design Methods
Wenbo Yu and Alexander D. MacKerell, Jr.
6. Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
Alexander Zipperer and Dorothee Kretschmer
Part II: Mode of Action
7. Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
Carolin Martina Kobras, Thorsten Mascher, and Susanne Gebhard
8. Determination of Bacterial Membrane Impairment by Antimicrobial Agents
Miriam Wilmes and Hans-Georg Sahl
9. Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes
Sebastian G. Hoß and Gerd Bendas
10. Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy
Kathi Scheinpflug, Oxana Krylova and Henrik Strahl
11. In vitro Assays to Identify Antibiotics Targeting DNA Metabolism
12. Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors