Antibiotics and Their Mechanisms of Action
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Antibiotics and Their Mechanisms of Action

Antibiotics and Their Mechanisms of Action


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About the Book

"Antibiotics and their mechanisms of action" provides a deep and lucid understanding of the mechanisms of action of various antibiotics. The experiments followed to decipher the mechanisms have been explained in great depth, which is a novelty in this textbook.

Most undergraduate textbooks on antibiotics place emphasis on their use from the clinician's perspective. Taking penicillin as an example, typical textbooks discuss its discovery, penicillin's β-lactam derivatives, routes of administration, the diseases and pathogens treated with

β-lactam antibiotics, side effects, and finally the mechanisms of resistance that have evolved against penicillin. No description of how its mechanism of action was deciphered is ever provided. This textbook describes how simple experiments using protoplasts, covalent affinity chromatography, SYPRO Orange binding assays, and X-ray crystallography have all been used to elucidate the mechanism of action of penicillin. Like this, the complicated mechanisms of action of different antibiotics have been presented in simple terms with the help of original illustrations. Chapters 8-10 cover the mechanisms of action of anti-ribosomal antibiotics (tetracycline, streptomycin, chloramphenicol). Elucidating the mechanisms of action for these antibiotics requires complex experiments such as ex vivo transcription via a cell-free ribosomal reaction mixture, photolabelling experiments, and sucrose density gradient centrifugation of radiolabelled translation reactants and products. These experiments are conveyed to the reader in the simplest possible language and with easily understandable, appealing illustrations. Finally, the reader is challenged at the end of every chapter with original questions, testing whether hecan apply the concepts he learned in the preceding chapter to research-level problems.

This book is written primarily for undergraduate students in microbiology or biotechnology courses to help them develop a simplistic yet comprehensive understanding of the mechanisms underlying antibiotics action. It is believed that this book will prepare the students for PhD level research.


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Product Details
  • ISBN-13: 9789819768509
  • Publisher: Springer Nature Singapore
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • ISBN-10: 9819768500
  • Publisher Date: 07 Nov 2024
  • Height: 235 mm
  • No of Pages: 126
  • Width: 155 mm


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