Part I: Properties, Design, Synthesis and Characterization of AMPs

1. Antimicrobial Peptides: An Introduction

Evan F. Haney, Sarah C. Mansour, and Robert E.W. Hancock

2. Tools for Designing Amphipathic Helical Antimicrobial Peptides

Davor Juretic, Damir Vukičevic, and Alessandro Tossi

3. Chemical Synthesis of Antimicrobial Peptides

Lena Münzker, Alberto Oddo and Paul R. Hansen

4. Microwave-Assisted Synthesis of Antimicrobial Peptides

Suhas Ramesh, Beatriz G. de la Torre, Fernando Albericio, Hendrik G. Kruger, and Thavendran Govender

5. High Performance Liquid Chromatography and Mass Spectrometry-Based Design of Protolytically Stable Antimicrobial Peptides

Mojtaba Bagheri and Robert E. W. Hancock

6. Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR

Reinhard Wimmer and Lars Erik Uggerhøj

7. Mass Spectrometry Approaches for Determining the Structure of Antimicrobial Peptides

Jiongyu Liu and Jianping Jiang

Part II: Studying Interactions of AMPs with Model Membranes and Bacteria

8. Molecular Dynamics Simulation and Analysis of the Antimicrobial Peptides-Lipid Bilayers Interactions

Shima Arasteh and Mojtaba Bagheri

9. Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides

Mauricio Arias, Elmar J. Prenner and Hans J. Vogel

10. Fluorescence and Absorbance Spectroscopy Methods to Study Membrane Perturbations by Antimicrobial Host Defense Peptides

Mauricio Arias and Hans J. Vogel

Kasper Kristensen, Jonas R. Henriksen, and Thomas L. Andresen

12. Macromolecule Biosynthesis Assay and Fluorescence Spectroscopy Methods to Explore Antimicrobial Peptide Mode(s) of Action

Bimal Jana, Kristin Renee Baker, and Luca Guardabassi

13. Using Confocal Microscopy and Computational Modeling to Investigate the Cell Penetrating Properties of Antimicrobial Peptides

Gabriel Del Rio, Edda Klipp, and Andreas Herrmann

14. Atomic Force Microscopy Study of the Interactions of Indolicidin with Model Membranes and DNA

Peter Fojan and Leonid Gurevich

15. Protocols for studying the interaction of MSI-78 with the Membranes of Whole Gram-positive and Gram-negative Bacteria by NMR

Nury P. Santisteban, M. R. Morrow, and V. Booth

16. Preparation of membrane models of Gram-negative bacteria and their interaction with Antimicrobial Peptides studied by CD and NMR

Rickey P. Hicks

17. Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells using Circular Dichroism

Concetta Avitabile, Luca Domenico D'Andrea and Alessandra Romanelli

18. Studying the Mechanism of Membrane Permeabilization induced by Antimicrobial Peptides using Patch-clamp Techniques

Giorgio Rispoli

19. Assays for Identifying Inducers of the Antimicrobial Peptide LL-37

Frank Nylen, Peter Bergman, Gudmundur H. Gudmundsson, and Birgitta Agerberth

20. Methods for Elucidating the Mechanism of Action of Proline-rich and other Non-lytic Antimicrobial Peptides

21. The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus Aureus Investigated by ATP-leakage, DNA-binding Analysis and the Expression of a LexA Controlled Gene, recA

Sanne Gottschalk and Line Elnif Thomsen

Part III: Assaying Selected