Part I: Human Immunodeficiency Virus Type 1 (HIV-1)

1. A Fluorescence-Based High Throughput Screening Assay to Identify HIV-1 Inhibitors

Peggy Geluykens, Koen Van Acker, Johan Vingerhoets, Christel Van den Eynde, Marnix Van Loock, and Géry Dams

2. A Homogeneous Time-Resolved Fluorescence Assay to Identify Inhibitors of HIV-1 Fusion

Liesbet Smeulders, Lieve Bunkens, Inge Vereycken, Koen Van Acker, Pascale Holemans, Emmanuel Gustin, Marnix Van Loock, and Géry Dams

3. Identification of HIV-1 Reverse Transcriptase Inhibitors Using a Scintillation Proximity Assay

Bertrand Van Schoubroeck, Marnix Van Loock, Tania Ivens, Pascale Dehertogh, Dirk Jochmans, and Géry Dams

4. Biochemical Screening Assays to Identify HIV-1 Integrase Inhibitors

Marleen Clynhens, Alexandra Smets, Inge Vereycken, Marnix Van Loock, Reginald Clayton, Geert Meersseman, and Olivia Goethals

5. HIV-1 Genotyping of the Protease-Reverse Transcriptase and Integrase Genes to Detect Mutations that Confer Anti-Retroviral Resistance

Peter Van den Eede, Liesbeth Van Wesenbeeck, Yvan Verlinden, Maxim Feyaerts, Veerle Smits, Ann Verheyen, Leen Vanhooren, Alain Deloof, Jorge Villacian, and Theresa Pattery

Part II: Hepatitis Viruses

6. In Vitro Kinetic Profiling of Hepatitis C Virus NS3 Protease Inhibitors by Progress Curve Analysis

Rumin Zhang and William T. Windsor

7. A Novel Hepatitis C Virus NS5B Polymerase Assay of De Novo Initiated RNA Synthesis Directed from a Heteropolymeric RNA Template

Eric Ferrari and Hsueh-Cheng Huang

8. Selecting and Characterizing Drug-Resistant Hepatitis C Virus Replicon

Inge Vliegen, Leen Delang, and Johan Neyts

9. A Cellular Replicon-Based Phenotyping Assay to Determine Susceptibility of Hepatitis C Virus Clinical Isolates to NS3/4A Protease Inhibitors

Leen Vijgen, Jannick Verbeeck, Barbara Van Kerckhove, Jan Martin Berke, Diana Koletzki, Gregory Fanning, and Oliver Lenz

10. Expression and Purification of Hepatitis C Virus Protease from Clinical Samples

Liesbet van der Helm

11. Surface Plasmon Resonance as a Tool to Select Potent Drug Candidates for Hepatitis C Virus NS5B Polymerase

Erna Cleiren, Benoit Devogelaere, and Katleen Fierens

12. Amplification and Sequencing of the Hepatitis C Virus NS3/4A Protease and the NS5B Polymerase Regions for Genotypic Resistance Detection of Clinical Isolates of Subtypes 1a and 1b

Diana Koletzki, Theresa Pattery, Bart Fevery, Leen Vanhooren, and Lieven J. Stuyver

13. A Southern Blot Assay for Detection of Hepatitis B Virus Covalently Closed Circular DNA from Cell Cultures

Dawei Cai, Hui Nie, Ran Yan, Ju-Tao Guo, Timothy M. Block, and Haitao Guo

14. In Vitro Phenotyping of Recombinant Hepatitis B Virus Containing the Polymerase/Reverse Transcriptase Gene from Clinical Isolates

Yang Liu and Kathryn M. Kitrinos

Part III: Flaviviruses

15. Cell-Based Antiviral Assays for Screening and Profiling Inhibitors Against Dengue Virus

Edwin Yunhao Gong, Marleen Clynhens, Tania Ivens, Pedro Lory, Kenny Simmen, and Guenter Kraus

16. A Duplex Real-Time RT-PCR Assay for Profiling Inhibitors of Four Dengue Serotypes

Edwin Yunhao Gong, Alexandra Smets, Nick Verheyen, Marleen Clynhens, Emmanuel Gustin, Pedro Lory, and Guenter Kraus

17. High Throughput Screening Using Dengue Virus Reporter Genomes

Wolfgang Fischl and Ralf Bartenschlager

18. Fluorimetric and HPLC-Based Dengue Virus Protease Assays Using a FRET Substrate

Christoph Ni