Approccio NGS per la scoperta di varianti nel gambero gigante d'acqua dolce
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Approccio NGS per la scoperta di varianti nel gambero gigante d'acqua dolce

Approccio NGS per la scoperta di varianti nel gambero gigante d'acqua dolce


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About the Book

Tra i crostacei d'allevamento, il gambero gigante d'acqua dolce Macrobrachium rosenbergii era fino a poco tempo fa una delle specie principali con un elevato valore commerciale. Ultimamente, ha perso popolarità a causa delle malattie e della crescita differenziata dei maschi a causa della gerarchia sociale. La specie può riacquistare il suo precedente status preferito se viene migliorata geneticamente per la crescita e la resistenza alle malattie. La selezione selettiva è notevolmente facilitata dalla selezione assistita da marcatori (MAS). I polimorfismi a singolo nucleotide (SNP) offrono la massima risoluzione possibile per una mappa di marcatori (un singolo cambiamento nucleotidico), sono codominanti, bi-allelici e generalmente abbondanti nelle popolazioni con un basso tasso di mutazione. Il sequenziamento del DNA attraverso piattaforme NGS è considerato l'approccio più economico. In questo studio è stata effettuata un'analisi degli SNP nelle sequenze trascritte di M. rosenbergii disponibili in GenBank, NCBI. Gli esemplari selvatici sono stati raccolti da diversi corpi idrici. Sono stati disegnati dei primer per amplificare la codifica (CDS) e sequenziati gli ampliconi su piattaforme GS-FLX 454 e Ion torrent. Sono stati rilevati 173 SNP nelle varie popolazioni. Questo approccio per l'estrazione di SNP nelle sequenze riportate è stato utilizzato da noi per la prima volta.


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Product Details
  • ISBN-13: 9786205086513
  • Publisher: KS Omniscriptum Publishing
  • Binding: Paperback
  • Language: Italian
  • Returnable: N
  • Weight: 195 gr
  • ISBN-10: 6205086514
  • Publisher Date: 18 Aug 2022
  • Height: 229 mm
  • No of Pages: 92
  • Spine Width: 6 mm
  • Width: 152 mm


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