1. Computational Design of RNA Libraries for in vitro Selection of Aptamers
Yaroslav Chushak, Jennifer A. Martin, Jorge L. Chávez, Nancy Kelley-Loughnane, and Morley O. Stone
2. In vitro Selection of RNA Aptamers for a Small-Molecule Dye
Asako Murata and Shin-ichi Sato
3. Development of Photoswitchable RNA Aptamer-Ligand Complexes
Gosuke Hayashi and Kazuhiko Nakatani
4. Identification of RNA Aptamers Against Recombinant Proteins with a Hexa-Histidine Tag
Shoji Ohuchi
5. Artificial Riboswitch Selection: A FACS-Based Approach
Zohaib Ghazi, Casey C. Fowler, and Yingfu Li
6. FRET-Based Optical Assay for Selection of Artificial Riboswitches
Svetlana V. Harbaugh, Molly E. Chapleau, Yaroslav G. Chushak, Morley O. Stone, and Nancy Kelley-Loughnane
7. Portable Two-Way Riboswitches: Design and Engineering
Ye Jin and Jian-Dong Huang
8. Generation of Orthogonally Selective Bacterial Riboswitches By Targeted Mutagenesis and in vivo Screening
Helen A. Vincent, Christopher J. Robinson, Ming-Cheng Wu, Neil Dixon, and Jason Micklefield
9. Dual Genetic Selection of Synthetic Riboswitches in Escherichia coli
Yoko Nomura and Yohei Yokobayashi
10. Nucleotide Kinase-Based Selection System for Genetic Switches
Kohei Ike and Daisuke Umeno
11. Measuring Riboswitch Activity in vitro and in Artificial Cells with Purified Transcription-Translation Machinery
Laura Martini and Sheref S. Mansy
12. Rational Design of Artificial ON-Riboswitches
Atsushi Ogawa
13. Engineering Protein-Responsive mRNA Switch in Mammalian Cells
Kei Endo and Hirohide Saito
14. Guanine-Tethered Antisense Oligonucleotides as Synthetic Riboregulators
Masaki Hagihara
15. In Vitro Selection of Allosteric Ribozymes that Sense the Bacterial Second Messenger c-di-GMP
Kazuhiro Furukawa, Hongzhou Gu, and Ronald R. Breaker
16. Dual-Selection for Evolution of in vivo Functional Aptazymes as Riboswitch Parts
Jonathan A. Goler, James M. Carothers, and Jay D. Keasling
17. In vivo Screening for Aptazyme-Based Bacterial Riboswitches
Charlotte Rehm and Jörg S. Hartig
18. Engineered Riboswitch as Gene-Regulatory Platform for Reducing Antibiotic Resistance
Libing Liu and Shu Wang
19. Construction of Ligand-Responsive microRNAs that Operate Through Inhibition of Drosha Processing
Chase L. Beisel, Ryan J. Bloom, and Christina D. Smolke
20. A Three-Dimensional Design Strategy for a Protein-Responsive shRNA Switch
Shunnichi Kashida and Hirohide Saito
