PART I: Atherosclerosis: Pathogenesis, model systems and emerging concepts.
1. Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions
Yee-Hung Chan and Dipak P. Ramji
2. Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation
Yee-Hung Chan and Dipak P. Ramji
3. Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis
Dipak P. Ramji, Alaa Ismail, Jing Chen, Fahad Alradi, and Sulaiman Al Alawi
4. Survey of approaches for investigation of atherosclerosis in vivo Dipak P. Ramji, Yee-Hung Chan, Alaa Alahmadi, Reem Alotibi, and Nouf Alshehri
5. Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis Juan Bautista Menendez-Gonzalez, and Neil P. Rodrigues
6. Peptides as therapeutic agents for atherosclerosis C. Roger White, Mayakonda Palgunachari, Paul Wolkowicz, and G. M. Anantharamaiah
PART II: In vitro and ex vivo model systems for atherosclerosis research. 7. Isolation and culturing of primary mouse and human macrophages
Alba de Juan and Begoña Lavin
8. Effective transfection and gene silencing of primary murine macrophages with small interfering RNA
Kim van Kuijk, Jan Nagenborg, and Judith C. Sluimer
9. Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques in vitro
Kerry S. Wadey, Alexandros Somos, Stephen J. Cross, Lien M. Reolizo, Jason L. Johnson, and Sarah J. George
10. Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis
Keman Xu, Fatma Saaoud, Stephanie Yu, Charles Drummer IV, Ying Shao, Yu Sun, Yifan Lu, Jianxin Sun, Jun Yu, Xiaohua Jiang, Hong Wang, and Xiaofeng Yang
11. Chick embryo ex vivo aortic sprouting assays for cardiovascular research
Selvaraj Vimalraj and Anuradha Dhanasekaran
12. Purification and analysis of circulating lipid particles
Barnaby W. R. Roper, Basmah Al-Sayejh, Ahmed Al-Aufi, Gary A. Cuthbert, Katie Lacey, Shervanthi Homer-Vanniasinkam, Michael A. Harrison, Darren C. Tomlinson, Ramzi Ajjan, and Sreenivasan Ponnambalam
13. Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research
Chandrakala Aluganti Narasimhulu and Sampath Parthasarathy
14. Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation
Alaa A. Alahmadi and Dipak P. Ramji
15. A fluorescent based in vitro method to assess cholesterol efflux. Sara Fernández-Castillejo, Anna Pedret, Úrsula Catalán, and Rosa Solà
16. Monitoring the anisotropy and fluidity of the HDL monolayer as
surrogates of HDL functionality
Sara Fernández-Castillejo, Anna Pedret, Úrsula Catalán, Claude Motta, and Rosa Solà
17. Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis
Andrea Rivas-Urbina, Noemi Rotllan, David Santos, Josep Julve, Joan Carles Escolà-Gil, and Jose Luis Sanchez-Quesada
18. Dual-fluorescence in vitro efferocytosis assay
Arif Yurdagul Jr
19. Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis
Yee-Hung Chan, and Dipak P. Ramji
20. P
