About the Book
1. Assaying Radiosensitivity of Ataxia-Telangiectasia
Hailiang Hu, Shareef Nahas, and Richard A. Gatti
2. Assaying for Radioresistant DNA Synthesis, the Hallmark Feature of the Intra-S-Phase Checkpoint Using a DNA Fiber Fluorography-Based Methodology
Amanda W. Kijas and Martin F. Lavin
3. ATM Gene Mutation Detection Techniques and Functional Analysis
Guillaume Rieunier, Catherine Dubois D'Enghien, Alice Fievet, Dorine Bellanger, Dominique Stoppa-Lyonnet, and Marc-Henri Stern
4. An HTRF(R) Assay for the Protein Kinase ATM
Phillip Adams, Jonathan Clark, Simon Hawdon, Jennifer Hill, and Andrew Plater
5. ATM Kinase Inhibitors: HTS Cellular Imaging Assay Using CellomicsTM ArrayScan VTI Platform
Catherine Bardelle and Joanna Boros
6. Image-Based High Content Screening: Automating the Quantification Process for DNA Damage-Induced Foci
Yi Chieh Lim
7. Analyzing ATM Function by Electroporation of Endonucleases and Immunofluorescence Microscopy
Keiji Suzuki
8. Quantitative and Dynamic Imaging of ATM Kinase Activity by Bioluminescence Imaging
Shyam Nyati, Grant Young, Brian Dale Ross, and Alnawaz Rehemtulla
9. Zn(II)-Phos-Tag SDS-PAGE for Separation and Detection of a DNA Damage-Related Signaling Large Phosphoprotein
Eiji Kinoshita, Emiko Kinoshita-Kikuta, and Tohru Koike
10. Identification of ATM Protein Kinase Phosphorylation Sites by Mass Spectrometry
Mark E. Graham, Martin F. Lavin, and Sergei V. Kozlov
11. Studies of ATM Kinase Activity Using Engineered ATM Sensitive to ATP Analogues (ATM-AS)
Masato Enari, Yuko Matsushima-Hibiya, Makoto Miyazaki, and Ryo Otomo
12. Functional Characterization of ATM Kinase Using Acetylation-Specific Antibodies
Sun Yingli and Du Fengxia
13. Identification of ATM-Interacting Proteins by Co-Immunoprecipitation and Glutathione-S-Transferase (GST) Pull-Down Assays
Amanda L. Bain, Janelle L. Harris, and Kum Kum Khanna
14. ATM Activation and H2AX Phosphorylation Induced by Genotoxic Agents Assessed by Flow- and Laser Scanning-Cytometry
Hong Zhao, H. Dorota Halicka, Jorge Garcia, Jiangwei Li, and Zbigniew Darzynkiewicz
15. Peptide Immunoaffinity Enrichment with Targeted Mass Spectrometry: Application to Quantification of ATM Kinase Phospho-Signaling
Jeffrey R. Whiteaker, Lei Zhao, Regine M. Schoenherr, Jacob J. Kennedy, Richard G. Ivey, and Amanda G. Paulovich
16. Mass Spectrometry-Based Proteomics for Quantifying DNA Damage-Induced Phosph
