1. Visualization and Measurement of Multiple Components of the Autophagy Flux
Tracey Evans, Robert Button, Oleg Anichtchik, and Shouqing Luo
2. Mitochondrial Redox Sensor for Drosophila Female Germline Stem Cells
Kiran Suhas Nilangekar and Bhupendra V. Shravage
3. Induction and Detection of Autophagy in Aged Hematopoietic Stem Cells by Exposing Them to Microvesicles Secreted by HSC-Supportive Mesenchymal Stromal Cells
Rohan S. Kulkarni, Manmohan S. Bajaj, and Vaijayanti P. Kale
4. Skeletal Muscle Lysosomal Function via Cathepsin Activity Measurement
Kristyn Gumpper, Matthew Sermersheim, Michael X. Zhu, and Pei-Hui Lin
5. Autophagy in Adipocyte Differentiation
Zhipeng Tao, Longhua Liu, Louise D. Zheng, and Zhiyong Cheng
6. Assessing Autophagy in the Leydig Cells
Hui Gao, Chao Liu, and Wei Li
7. Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells
Sinem Tunçer and Sreeparna Banerjee
8. Simultaneous Detection of Autophagy and Epithelial to Mesenchymal Transition in the Non-Small Cell Lung Cancer Cells
Javad Alizadeh, Shahla Shojaei, Adel Sepanjnia, Mohammad Hashemi, Eftekhar Eftekharpour, and Saeid Ghavami
9. Autophagy in Zebrafish Extraocular Muscle Regeneration
Alfonso Saera-Vila, Phillip E. Kish, and Alon Kahana
10. The Detection Techniques for Autophagy-Associated Cell Death Related Genes and Proteins: Gene Expression Assay and Immunohistochemistry
Melek Ozturk, Ozlem Dagdeviren Ozsoylemez, and Fatma Kaya Dagistanli
11. Cloning of Autophagy-Related MicroRNAs
Deniz Gulfem Ozturk, Muhammed Kocak, and Devrim Gozuacik
12. Assays to Monitor Aggrephagy in Drosophila Brain
Anne-Claire Jacomin and Ioannis P. Nezis
13. Methods for Monitoring Autophagy in Silkworm Organs
Aurora Montali, Morena Casartelli, Daniele Bruno, Annalisa Grimaldi, and Gianluca Tettamanti
14. Immunofluorescence Staining Protocols for Major Autophagy Proteins Including LC3, P62, ULK1 in Mammalian Cells in Response to Normoxia and Hypoxia
Wen Li, Shupeng Li, Yifang Li, Xiaoying Lin, Yongquan Hu, Tian Meng, Baojin Wu, Rongrong He, and Du Feng
15. Identification of Novel Autophagy Inhibitors via Cell-Based High-Content Screening
Georgios Konstantinidis, Sonja Sievers, and Yao-Wen Wu
16. Porcine Cell-Free System to Study Mammalian Sperm Mitophagy
Won-Hee Song and Peter S
