Part I: Review
1. Axon Regeneration: What Needs to Be Overcome?
Andrew J. Murray
Part II: Monitoring Intracellular Pathways in Growth Cones
2. Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro
Hiroki Akiyama and Hiroyuki Kamiguchi
3. The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance Related Intracellular Signaling in Live Dorsal Root Ganglia Neuronal Growth Cones
Steven J. Tucker
Part III: Assessing Axonal Responses to Extracellular Factors In Vitro
4. Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues
Andrew Kaplan, Ricardo Sanz, Gino B. Ferraro, Ricardo Alchini, and Alyson E. Fournier
5. Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants
Tom Buyens, Djoere Gaublomme, Inge Van Hove, Lies De Groef, and Lieve Moons
6. Growth Cone Collapse Assay
Geoffrey M.W. Cook, Prem Jareonsettasin, and Roger J. Keynes
7. Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study
Jaewon Park, Sunja Kim, Jianrong Li, and Arum Han
8. Organotypic Slice Co-Culture Systems to Study Axon Regeneration in the Dopaminergic System Ex Vivo
Claudia Heine and Heike Franke
9. Monitoring Neuron and Astrocyte Interactions with a 3D Cell Culture System
James B. Phillips
Part IV: Axon Injury and Regeneration In Vivo
10. Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury
Jessica C.F. Kwok, Janosch P. Heller, Rong-Rong Zhao, and James W. Fawcett
11. Polymeric Biomaterials for Nerve Regeneration: Fabrication and Implantation of a Biodegradable Nerve Guide
Wesley N. Sivak, Jacqueline M. Bliley, and Kacey G. Marra
12. A Highly Reproducible Mouse Model of Compression Spinal Cord Injury
Suelen Adriani Marques, Fernanda Martins de Almeida, Klauss Mostacada, and Ana Maria Blanco Martinez
13. Using Templated Agarose Scaffolds to Promote Axon Regeneration Through Sites of Spinal Cord Injury
Jacob Koffler, Ramsey F. Samara, and Ephron S. Rosenzweig
14. In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration
Saijilafu, Bo-Yin Zhang, and Feng-Quan Zhou
Part V: Assessing the Extent of Axon Regeneration
15. Assessing Motor Outcome and Functional Recovery Following Nerve Injury
Ronald Deumens, Claudia Marinangeli, Ahmet Bozkurt, and Gary Anthony Brook
16. The Use of an Adeno-Associated Viral Vector for Efficient Bicistronic Expression of Two Genes in the Central Nervous System
Thomas Haynes Hutson, Claudia Kathe, Sean Christopher Menezes, Marie-Claire Rooney, Hansruedi Bueler, and Lawrence David Falcon Moon
17. Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues
Xueting Luo, Benjamin Yungher, and Kevin K. Park
18. Time-Lapse In Vivo Imaging of Dorsal Root Nerve Regeneration in Mice
Andrew Skuba, Meredith Ann Manire, Hyukmin Kim, Seung Baek Han, and Young-Jin Son
19. Using Manganese-Enhanced MRI to Assess Optic Nerve Regeneration
Ioanna Sandvig and Axel Sandvig
