Part I: BAC Construction and Characterization

1 From Selective Full-Length Genes Isolation By TAR Cloning In Yeast To Their Expression From HAC Vectors In Human Cells

2 Recombineering Linear BACs

Qingwen Chen and Kumaran Narayanan

3 BAC Sequencing Using Pooled Methods

Chris A. Saski, F. Alex Feltus, Laxmi Parida, and Niina Haiminen

Part II: Modification Of BACs

4 Making BAC Transgene Constructs With Lambda-Red Recombineering System

For Transgenic Animals Or Cell Lines

Scott Holmes, Suzanne Lyman, Jen-Kang Hsu, and Jrgang Cheng

5 Directing Enhancer-Traps And Itol2 End Sequences To Deleted BAC Ends With Loxp- and Lox511-Tn10 Transposons

Pradeep K. Chatterjee

6 Recombining Overlapping BACs Into Single Large BACs

George Kotzamanis and Athanassios Kotsinas

Part III: Gene Expression Using BACs as Vectors

7 Generation Of Knockout Alleles by RFLP Based BAC Targeting Of Polymorphic Embryonic Stem Cells

Tahsin Stefan Barakat and Joost Gribnau

8 Herpesvirus Mutagenesis Facilitated By Infectious Bacterial Artificial Chromosomes (Ibacs)

Karl E. Robinson and Timothy J. Mahony

9 Conversion Of BAC Clones Into Binary BAC (BIBAC) Vectors and Their Delivery Into Basidiomycete Fungal Cells Using Agrobacterium tumefaciens

Shawkat Ali and Guus Bakkeren

10 Infectious Delivery of Alphaherpesvirus Bacterial Artificial Chromosomes

Kurt Tobler and Cornel Fraefel

Part IV: Applications Of BACs In Model Organisms, Medical Genetics, and Drug Discovery

11 A Recombineering-Based Gene Tagging System For Arabidopsis

Jose M. Alonso and Anna N. Stepanova

12 BAC Transgenic Zebrafish for Transcriptional Promoter and Enhancer Studies

Petra Kraus, Cecilia L Winata, and Thomas Lufkin

13 Bacs-On-Beads^TM (Bobs^TM) Assay for the Genetic Evaluation of Prenatal Samples And Products of Conception

Francesca Romana Grati, François Vialard, and Susan Gross

14 New BAC Probe Set To Narrow Down Chromosomal Breakpoints In Small and Large Derivative Chromosomes, Especially Suited for Mosaic Conditions

Ahmed B. Hamid, Xiaobo Fan, Nadezda Kosyakova, Gopakumar Radhakrishnan, Thomas Liehr, and Tatyana Karamysheva

15 BAC-Probes Applied For Characterization Of Fragile Sites (FS)

Kristin Mrasek, Kathleen Wilhelm, Luciana G. Quintana, Luise Theuss, Thomas Liehr, Andreja Leskovac, Jelena Filipovic, Gordana Joksic, Ivana Joksic, and Anja Weise

16 Application Of BAC-Probes To Visualize Copy Number Variants (CNVs)

Anja Weise, Moneeb A.K. Othman, Samarth Bhatt, Sharon Löhmer, and Thomas Liehr

17 Site-Specific Integration Of Bacterial Artificial Chromosomes Into Human Cells Bradley Mccoll, Sara Howden, and Jim Vadolas

18 Generation Of BAC Reporter Cell Lines For Drug Discovery

Betty R. Kao, Bradley Mccoll, and Jim Vadolas