Bacterial Pathogenesis
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Bacterial Pathogenesis: Methods and Protocols

Bacterial Pathogenesis: Methods and Protocols

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About the Book

Bacterial infections affect world health today as a leading cause of morbidity and mortality. Pathogenic bacteria routinely command a broad spectrum of niches in the human host, making an understanding of pathogenesis mechanisms crucial to the development of prophylactics and treatment for bacterial diseases. A variety of in vitro methods, in vivo animal model systems and cutting-edge genomics assays have arisen in the effort to study bacterial pathogenesis and identify potential therapeutic targets. In Bacterial Pathogenesis, in-depth methods and state-of-the-art protocols are presented for investigating specific mechanisms of pathogenesis for a wide range of bacteria. This invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections. Up-to-date molecular typing methods for Staphylococcus aureus and a new model of streptococcal pharyngitis in non-human primates are also included. Bacterial Pathogenesis will prove an invaluable collection for microbiologists, immunologists, cell biologists and infectious disease clinicians - and indispensable to all science researchers interested in studying pathogenic bacteria and related disease processes.

Table of Contents:
Preface.- Contributors.- List of Color Plates.- I: Mechanisms of Bacterial Pathogenesis.- 1 Isolation and Characterization of LipopolysaccharidesMichael A. Apicella.- 2 Proteomic Analysis of Proteins Secreted by Streptococcus pyogenesMichelle A. Chaussee, Emily J. McDowell, and Michael S. Chaussee.- 3 Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureusSusanne Engelmann and Michael Hecker.- 4 Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and EvolutionCaitriona M. Guinane and J. Ross Fitzgerald.- 5 Detection and Inhibition of Bacterial Cell–Cell CommunicationScott A. Rice, Diane McDougald, Michael Givskov, and Staffan Kjelleberg.- 6 A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsiiJames M. Battisti, Sandra J. Raffel, and Tom G. Schwan.- 7 Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferiPhilip E. Stewart and Patricia A. Rosa.- 8 The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin?A Molecular and Biochemical ApproachCuong Vuong and Michael Otto.- II: Host-Pathogen Interaction.- 9 Analysis of Staphylococcus aureus Gene Expression During PMN PhagocytosisJovanka M. Voyich, Dan E. Sturdevant, and Frank R. DeLeo.- 10 Examining the Vector–Host–Pathogen Interface With Quantitative Molecular ToolsJason E. Comer, Ellen A. Lorange, and B. Joseph Hinnebusch.- 11 Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine MacrophagesJean Celli.- 12 Rate and Extent of Helicobacter pylori PhagocytosisLee-Ann H. Allen.- 13 Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human NeutrophilsDori L. Borjesson.- 14 Ultrastructural Analysis ofBacteria–Host Cell InteractionsDavid W. Dorward.- 15 Infection of Human Monocyte-Derived Macrophages With Coxiella burnetiiJeffrey G. Shannon and Robert A. Heinzen.- 16 Infection of Epithelial Cells With Salmonella entericaOlivia Steele-Mortimer.- 17 Determining the Cellular Targets of Reactive Oxygen Species in Borrelia burgdorferiJulie A. Boylan and Frank C. Gherardini.- III: Animal Models of Bacterial Infection.- 18 Bioluminescent Imaging of Bacterial Biofilm Infections In VivoJagath L. Kadurugamuwa and Kevin P. Francis.- 19 The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans: Cotton Rat S. aureus Nasal Colonization ModelJohn F. Kokai-Kun.- 20 A Non-Human Primate Model of Acute Group A Streptococcus PharyngitisPaul Sumby, Anne H. Tart, and James M. Musser.- IV: Identification of Therapeutic Targets and Typing Methods.- 21 Target-Based Antimicrobial Drug DiscoveryLefa E. Alksne and Paul M. Dunman.- 21 Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa TypingBarun Mathema, Jose Mediavilla, and Barry N. Kreiswirth.


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Product Details
  • ISBN-13: 9781588297402
  • Publisher: Humana Press Inc.
  • Publisher Imprint: Humana Press Inc.
  • Height: 235 mm
  • No of Pages: 320
  • Sub Title: Methods and Protocols
  • ISBN-10: 1588297403
  • Publisher Date: 31 Jan 2008
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Width: 155 mm


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