PART I: Introduction 1. Studying [NV1] Bacterial Persistence: Established Methods And Current Advances
Elen Louwagie, Laure Verstraete, Jan Michiels, and Natalie Verstraeten
PART II: Quantification and enrichment of persister cells 2. Antibiotic Tolerance and Persistence Studied Throughout Bacterial Growth Phases
Enea Maffei, Cinzia Fino, and Alexander Harms
3. A Robust Method For Generating, Quantifying, and Testing Large Numbers of Escherichia coli persisters
Silke R. Vedelaar, Jakub L. Radzikowski, and Matthias Heinemann
4. Enrichment of Persister Cells through β-lactam-induced Filamentation and Size Separation Etthel Windels, Bram Van den Bergh, and Jan Michiels
5. Methods for Enrichment of Bacterial Persister Populations For Phenotypic Screens And Genomic Studies
Samantha Adikari, Elizabeth Hong-Geller, and Sofiya Micheva-Viteva
PART III: Single-cell analysis of persister cells 6. Observing Bacterial Persistence at Single-Cell Resolution
Emma Dawson, Emrah Şimşek, and Minsu Kim
7. Phenotypic Characterization of Antibiotic Persisters at the Single-Cell Level: From Data Acquisition to Data Analysis
Nathan Fraikin, Laurence Van Melderen, and Frédéric Goormaghtigh
8. Microfluidics for Single-Cell Study of Antibiotic Tolerance and Persistence Induced by Nutrient Limitation
Stefany Moreno-Gámez, Alma Dal Co, Simon van Vliet, and Martin Ackermann
9. Counting Chromosomes in Individual Bacteria to Quantify their Impacts on Persistence
Allison M. Murawski, Katherine Rittenbach, Christina J. DeCoste, Gary Laevsky, and Mark P. Brynildsen PART IV: Omics approaches in persistence research 10. Analyzing PERSISTER PROTEOMES with SILAC and Label-Free Methods
Bork A. Berghoff 11. Defining Proteomic Signatures To Predict Multi-Drug Persistence in Pseudomonas aeruginosa
Pablo Manfredi, Isabella Santi, Enea Maffei, Emmanuelle Lezan, Alexander Schmidt, and Urs Jenal
12. The use of Experimental Evolution to Study The Response of Pseudomonas aeruginosa to Single or Double Antibiotic Treatment
Isabella Santi, Pablo Manfredi, Urs Jenal
PART V: Persister cell resuscitation and eradication 13. Detecting persister awakening determinants
Dorien Wilmaerts, Jan Michiels, and Natalie Verstraeten
14. Monitoring Persister Resuscitation with Flow Cytometry
Sayed Golam Mohiuddin and Mehmet A. Orman
15. Stimulating Aminoglycoside Uptake to Kill Staphylococcus aureus persisters
Ashelyn E. Sidders, Lauren C. Radlinski, Sarah E. Rowe, and Brian P. Conlon
PART VI: Cellular and animal model systems for studying persistence 16. In vitro Models for the Study of the Intracellular Activity Of Antibiotics
Frédéric Peyrusson, Tiep K. Nguyen, Julien M. Buyck, Sandrine Lemaire, Gang W. Wang, Cristina Seral, Paul M. Tulkens, and Van Bambeke
17. Analysis of Salmonella Persister Population Sizes, Dynamics Of Gut Luminal Seeding, And Plasmid Transfer In Mouse Models Of Salmonellosis
Erik Bakkeren, Joshua P. M. Newson, and Wolf-Dietrich Hardt
18. Studying Antibiotic Persistence During Infection
Charlotte Michaux, Severin Ronneau, and Sophie Helaine
