Beta-Arrestins

Preface...
Table of Contents...
Contributing Authors...

Part I Introduction

1. A Brief History of the b-Arrestins
Marc G. Caron and Lawrence S. Barak

Part II Methods to Study Roles of b-Arrestins in GPCR Regulation

3. Methods to Monitor the Trafficking of β-Arrestin/G Protein-Coupled Receptor Complexes using Enhanced Bystander BRET
Yubo Cao, Yoon Namkung, and Stéphane A. Laporte

4. Methods to Investigate b-Arrestin-Mediated Regulation of GPCR Function in Human Airway Smooth Muscle
Tonio Pera and Raymond B. Penn

5. Measuring Recruitment of b-Arrestin to G Protein-Coupled Heterodimers using Bioluminescence Resonance Energy Transfer
Dany Fillion, Dominic Devost, and Terence E. Hébert

6. Detection of β-Arrestin-Mediated G Protein-Coupled Receptor Ubiquitination using BRET
Karim Nagi and Sudha K. Shenoy

Part III Methods to Study Scaffolding Functions of b-Arrestins

7. Using In Vitro Pull-Down and In Cell Overexpression Assays to Study Protein Interactions with Arrestin
Nicole A. Perry, Xuanzhi Zhan, Eugenia V. Gurevich, T. M. Iverson, and Vsevolod V. Gurevich

8. Methods to Investigate Arrestins in Complex with Phosphodiesterases
Amy Tibbo, Tara Busiau, and George S. Baillie

9. Methods to Characterize Protein Interactions with β-arrestin in cellulo
Revu Ann Alexander, Isaure Lot, and Hervé Enslen

10. Methods to Investigate the β-Arrestin-Mediated control of ARF6 Activation to Regulate Trafficking and Actin Cytoskeleton Remodeling
Ricardo Charles, Mohamed Bourmoum, Shirley Campbell, and Audrey Claing

11. Methods to Investigate the Roles of b-Arrestin-Dependent RalGDS Activation in GPCR-Stimulated Membrane Blebbing
Stephen S. G. Ferguson

12. Methods to Determine Interaction Interfaces between b-Arrestins and Their Protein Partners
Thomas Bourquard, Astrid Musnier, Aurélie Tréfier, Flavie Landomiel, Thomas Boulo, Eric Reiter, Pascale Crépieux, and Anne Poupon

13. Workflow Description to Dynamically Model β-Arrestin Signaling Networks
Romain Yvinec, Mohammed Akli Ayoub, Francesco De Pascali, Pascale Crépieux, Eric Reiter, and Anne Poupon

14. Proteomic Analysis of the β-Arrestin Interactome
Yang Zhao and Kunhong Xiao

Part IV Methods to Analyse Ligand Bias

Part V Methods to Study b-Arrestin Localization

16. Methods to Investigate the Nucleocytoplasmic Shuttling Properties of b-Arrestins
Elodie Blondel-Tepaz, Thomas Guilbert, and Mark G.H. Scott

17. Monitoring β-Arrestin2 Targeting to the Centrosome, Basal Body and Primary Cilium by Fluorescence Microscopy
Anahi Molla-Herman, Kathryn M.