Step by Step Technical Manual of Blood Components Preparation
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Step by Step Technical Manual of Blood Components Preparation

Step by Step Technical Manual of Blood Components Preparation


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About the Book

This book covers all areas of the subject, i.e. selection of donors, safe handling of equipment, calibration, preparation of components, component storage, component thawing, issue of component, transfusion doses, quality control, etc.
Important information is presented clearly for quality product.
Ready reference for safe and qualitative preparation of blood components.
This is useful for doctors, technicians, nurses, and a person who donates blood.

About the Author

Bibekananda Mukherjee BSc DMLT WHO Fellow



Table of Contents:
Contents Chapter 1 : Introduction 1 • What is better—Whole blood or components? 1 Chapter 2 : Definition of Blood Components 3 • Functions of blood components 3 Chapter 3 : Blood Components Separation 5 • Introduction 5 Chapter 4 : Benefits and Advantages of Blood Components 7 • The following components are benefited 8 Chapter 5 : Methodology of Blood Components Preparation 10 • Donor selection 10 • Blood collection techniques for donor 11 • General awareness and precaution for donor 14 • Care after donation 15 • Health benefits of donating blood 17 • Defer the donor temporarily 18 • Restricted person for blood donation 19 • Blood group choice for blood and blood components 21 • ABO blood group systems 24 • Safe plasma transfusion 24 • Common blood type in Indian people 24 • Bag selection 25 • Selection of bag for donating blood 27 • Precautions required for blood components preparation area 28 • Precautions required for blood components preparation 30 xx Step by Step Technical Manual of Blood Components Preparation • Common things to be done before preparation of blood components 31 • Common things to be done during preparation of blood components 35 Chapter 6 : Basic Informations for Blood Components Product 37 • Instruction followed for blood components preparation 37 • Materials required for blood components product 41 Chapter 7 : Safe Handling of Equipments 44 • Refrigerated centrifuge (Cryofuge 6000i) 45 • Programing of cryofuge 6000i 45 • Handling of cryofuge centrifuge 6000i and other models of refrigerated centrifuge 46 • Check calibration of cryofuge centrifuge 6000i and other models of refrigerated centrifuge 48 • Horizontal laminar airflow bench 53 • Circulating plasma/cryowater bath 54 • Handling of circulating plasma/ cryowater bath 55 • Electronic sealer 55 • Operation of electronic sealer 55 Chapter 8 : Preparation of Blood Components— Procedures 57 • Blood components preparation 57 • Packed red blood cell (PRBC)/fresh frozen plasma (FFP) by double bags collection 58 • Leuko-depleted packed cell/fresh frozen plasma by triple bags collection 63 • Packed red blood cell (PRBC)/ cryoprecipitate/cryopoor plasma by triple bags collection 65 • Cryoprecipitate component preparation 66 Contents xxi • Packed red blood cell (PRBC)/platelets concentrate/platelets-poor plasma/Fresh frozen plasma (PPP/FFP) by random donor in triple bags collection 69 • Packed red blood cell(PRBC)(leuko-depleted)/ platelets concentrate by (buffy-coat method)/ FFP preparation inquadruple bags 71 • Instruction follows after preparation of platelet concentrate in triple bags and quadruple bags 74 • Leuko-depleted packed cell, FFP and cryoprecipitate component preparation by quadruple bags 78 • Packed red blood cell (leuko-depleted), fresh frozen plasma/cryoprecipitate by quadruple bags (SAGM-2/adsol pre- servative) 81 • Packed red blood cell (leuko-depleted)/ platelets concentrate by (PRP method) and platelet-poor plasma (PPP/ FFP) in quadruple bags 84 • Packed red blood cell (PRBC) /platelets concentrate by (PRP method)/platelet- poor plasma (PPP/FFP)/cryoprecipitate or required amount of FFP for pediatrics patient in quadruple bags 87 • Saline-washed packed red blood cell (PRBC) in double bags preparation (450 mL/350 mL) 90 Chapter 9 : Preservation and Distribution of Blood Components 94 • Storage of blood components 94 • Thawing procedure 94 xxii Step by Step Technical Manual of Blood Components Preparation • Procedure to issue components 96 Chapter 10 : Component Transfusion´s Dose and Rate of Infusion 98 • Fresh frozen plasma 98 • Platelets concentrate 99 • Cryoprecipitate 100 • Infusion rates of various components 102 Chapter 11 : Quality Control 103 • Sterility tests 103 • Quality control specifications and procedures for blood components 104 Chapter 12 : Biomedical Waste Management 108 • Biomedical waste 108 • Biomedical waste management in relation to blood banking activities 110 • Preparation of sodium hypochlorite solution 112 Chapter 13 : Disinfection Protocol 113 • Procedure 113 Chapter 14 : Sterilization 115 • Autoclave (sterilizer) 115 Chapter 15 : Blood Bank Refrigerator 118 Further Reading 121 Index 123


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Product Details
  • ISBN-13: 9789351526049
  • Publisher: Jaypee Brothers Medical Publishers Pvt. Ltd.
  • Publisher Imprint: Jaypee
  • Edition: 1
  • Language: English
  • Width: 121 mm
  • ISBN-10: 9351526046
  • Publisher Date: 2016
  • Binding: Paperback
  • Height: 178 mm
  • No of Pages: 160


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Step by Step Technical Manual of Blood Components Preparation
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