1. Library Construction for Mutation Identification by Whole-Genome Sequencing
Harold E. Smith
2. Fundamentals of Comparative Genome Analysis in Caenorhabditis Nematodes
Eric S. Haag and Cristel G. Thomas
3. Genetic Methods for Cellular Manipulations in C. elegans
Menachem Katz
4. A Fusion PCR Method for Expressing Genetic Tools in C. elegans
Yifat Eliezer and Alon Zaslaver
5. Transposon Assisted Genetic Engineering with Mos1 Mediated Single-copy Insertion (MosSCI)Christian Frøkjær-Jensen
6. Creating Genome Modifications in C. elegans using the CRISPR/Cas9 SystemJohn A. Calarco and Ari E. Friedland
7. Observing and Quantifying Fluorescent Reporters
Michael Hendricks
8. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior
Caspar Glock, Jatin Nagpal, Alexander Gottschalk
9. Simultaneous Optogenetic Stimulation of Individual Pharyngeal Neurons and Monitoring of Feeding Behavior in Intact C. elegans
Nicholas F Trojanowski and Christopher Fang-Yen
10. High-Pressure Freeze and Freeze Substitution Electron Microscopy in C. elegansLaura Manning and Janet Richmond
11. Electron Tomography Methods for C. elegans
David H. Hall and William J. Rice
12. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans
Ross C. Lagoy and Dirk R. Albrecht
13. Tracking Single C. elegans using a USB Microscope on a Motorized Stage
Eviatar I. Yemini and André E.X. Brown
14. An Imaging System for C. elegans Behavior
Matthew A. Churgin and Christopher Fang-Yen
15. A Method for Obtaining Large Populations of Synchronized Caenorhabditis elegans dauer Larvae
Maria C. Ow and Sarah E. Hall
16. Sampling and Isolation of C. elegans from the Natural Habitat
Nausicaa Poullet and Christian Braendle
17. A Primer on Prototyping
Dylan Lynch and David Biron
18. A Primer on Quantitative Modeling
Iulia Neagu and Erel Levine
