Calcium-Binding Proteins of the Ef-Hand Superfamily

Part I: Data Analysis and Modern Technologies to Study Calcium-Binding and Signaling in Cells

1. Using Data Mining to Explore Calmodulin Bibliography

Jacques Haiech and Marie-Claude Kilhoffer

2. Deep Two-Photon Imaging In Vivo with a Red-Shifted Calcium Indicator

Antje Birkner and Arthur Konnerth

3. High Resolution Calcium Imaging Method for Local Calcium Signaling

Björn-Philipp Diercks, René Werner, Daniel Schetelig, Insa M.A. Wolf, and Andreas H. Guse

4. Measurement of Contractility and Calcium Release in Cardiac Spheroids

Christian Zuppinger

5. Simultaneous Recording of Subcellular Ca²⁺ Signals from the Cytosol and Sarco/Endoplasmatic Reticulum: Compartmentalized Dye Loading, Imaging, and Analysis

Ernst Niggli and Miguel Fernandez-Tenorio

6. The Use of Complementary Luminescent and Fluorescent Techniques for Imaging Ca²⁺ Signalling Events during the Early Development of Zebrafish (Danio rerio)

Sarah E. Webb and Andrew L. Miller

7. Cellular Ca²⁺-Responding Nanoluciferase Reporter Gene System Directed by Tandemly Repeated Pseudo-Palindromic NFAT-Response Elements

Wei Zhang, Terunao Takahara, Takuya Achiha, Hideki Shibata, and Masatoshi Maki

8. Designing Calcium Binding Proteins for Molecular MR Imaging

Mani Salarian, Shenghui Xue, Oluwatosin Y. Ibhagui, and Jenny J. Yang

9. Coordination to Divalent Cations by Calcium Binding Proteins

10. Chaperoning against Amyloid Aggregation: Monitoring In Vitro and In Vivo

Ravichandran Vignesh and Gopala Krishna Aradhyam

Part II: The Superfamily of Calcium-Binding Proteins Characterized by the EF-Hand Structural Motif

11. Ca²⁺-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets

Claus W. Heizmann

12. Gene Transfer of Calcium Binding Proteins into Adult Cardiac Myocytes

Brian R. Thompson, Houda Cohen, Addeli Bez Batti Angulski, and Joseph M. Metzger

13. Expression and Purification of Calmodulin for NMR and Other Biophysical Applications

Benjamin Grant, Chris Marshall, and Mitsu Ikura

14. The Use of Cre-loxP Inducible Mouse Models to Dissect the Specific Roles of Calcineurin Signalling in Myeloid Cells

Teresa Zelante and Jan Frič

15. Calpain Purification through Calpastatin and Calcium: Strategy and Procedures

Hung Huy Nguyen,