About the Book
Preface...
Table of Contents...
Contributing Authors...
1. Usage of Droplet Digital PCR (ddPCR) Assays for T Cell Quantification in Cancer
Willem H. Zoutman, Rogier J. Nell, and Pieter A. van der Velden
2. An Economical, Quantitative, and Robust Protocol for High Throughput T Cell Receptor Sequencing from Tumor or Blood
Imran Uddin, Kroopa Joshi, Theres Oakes, James M. Heather, Charles Swanton on behalf of the TRACERx consortiumx, and Benny Chain
3. Generation of Tumor Antigen-Specific Cytotoxic T Lymphocytes from Pluripotent Stem Cells
Xiaoniao Chen, Fengyang Lei, Liqiang Wang, Xiaofang Xiong, and Jianxun Song
4. Expansion and Adoptive Transfer of Human Vδ2+ T Cells to Assess Anti-Tumor Effects In Vivo
Akshat Sharma, Nicholas A. Zumwalde, and Jenny E. Gumperz
5. Usage of Single-Cell RNA-Sequencing to Unveil Immune Lymphoid Cell Precursors
Yong Yu and Pentao Liu
6. Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance
Monica Parodi, Raffaella Meazza, Chiara Vitale, Gabriella Pietra, Paolo Carrega, and Massimo Vitale
7. CD107a Degranulation Assay to Evaluate Immune Cell Anti-Tumor ActivitySeila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, and Alejandro López-Soto
8. A Flow Cytometric NK Cell-Mediated Cytotoxicity Assay to Evaluate Anti-Cancer Immune Responses In Vitro
Seila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo Gonzalez, and Alejandro López-Soto
9. Mouse Models to Study Natural Killer Cell-Mediated Immunosurveillance and Metastatic Latency
Vidhya R. Nair and Srinivas Malladi
10. Tumor-Associated Macrophage Isolation and In Vivo Analysis of their Tumor Promoting Activity
Nicholas M. Clark and Paula D. Bos
11. Conditional Genetic Ablation Mouse Models as a Tool to Study Cancer Immunosurveillance In Vivo
Magali Dupont and Christian A.J. Vosshenrich
12. Determination and Isolation of Immune Populations from Brain Tumor Microenvironments
Javier Godino and Alberto J. Schuhmacher
13. Mouse Model of Colitis Associated Colorectal Cancer (CAC): Isolation and Characterization of Mucosal-Associated Lymphoid Cells
Llipsy Santiago, Marta Castro, Julián Pardo, and Maykel Arias
14. Cancer Exome-Based Identification of Tumor Neo-Antigens Using Mass Spectrometry
Shelly Kalaora and Yardena Samuels
15. Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways
Cherie' R. Scurrah, Alan J. Simmons, and Ken S. Lau
16. Purification of Leukemia-Derived Exosomes to Study Microenvironment Modulation
Marina Wierz, Sandrine Pierson, Ernesto Gargiulo, Coralie Guerin, Etienne Moussay, and Jerome Paggetti
17. Molecular Characterization of Circulating Tumor Cells to Study Cancer Immunoevasion
Chiara Nicolazzo, Angela Gradilone, Guido Carpino, Paola Gazzaniga, and Cristina Raimondi
18. Quantitative Identification of Senescent Cells in Cancer
Anat Biran, Ziv Porat, and Valery Krizhanovsky
19. Generation and Harnessing of Heterotypic Tumor-Stroma Spheroids to Study Cancer Immunosurveillance
Dirk Zboralski and Anna Frömming
20. Establishment of Slice Cultures as a Tool to Study the Cancer Immune Microenvironment
Xiuyun Jiang, Y. David Seo, Kevin Sullivan, and Venu G. Pillarisetty
21. Gold Standard Assessment of Immunogenic Cell Death in Oncological Mouse Models
Juliette Humeau, Sarah Lévesque, Guido Kroemer, and Jonathan G. Pol
22. A Novel Dendritic Cell-Based Vaccination Protocol to Stimulate Immunosurveillance of Aggressive Cancers
Annunziata Nigro, Barbara Montico, Vincenzo Casolaro, and Jessica Dal Col
23. Development of Bi-Specific Antibody Derivatives for Cancer Immunotherapy
Yuan He, Wijnand Helfrich, and Edwin Bremer
24. Generation of CAR-T Cells f
