1 Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction
Daniel R. Crooks, Teresa W-M. Fan, and W. Marston Linehan
2 Imaging Cancer Metabolism with Positron Emission Tomography (PET)
Timothy H. Witney and David Y. Lewis
3 Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells
Debanti Sengupta and Guillem Pratx
4 Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics
Tong Zhang, Christiaan F. Labuschagne, Karen H. Vousden, and Oliver D. K. Maddocks
5 Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM)
Sarah Creed and Matthew McKenzie
6 Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells
David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, and Carmen Chak-Lui Wong
7 Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment
Ioannis S. Pateras and Tomer Cooks
8 Quantitation of Macropinocytosis in Cancer Cells
Koen M.O. Galenkamp, Basheer Alas, and Cosimo Commisso
9 Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation
Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, and Alessandro Carrer
10 Methods to Measure Autophagy in Cancer Metabolism
Natalia Von Muhlinen
11 Lipidomic Analysis of Cancer Cell and Tumor Tissue
Sk Ramiz Islam and Soumen Kanti Manna
12 Mass Spectrometry-based Profiling of Metabolites in Human Biofluids
Tanushree Chakraborty and Soumen Kanti Manna
13 Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites Jodi Rattner, Farshad Farshidfar, and Oliver F. Bathe
14 Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy
Beata Mickiewicz, M. Eric Hyndman and Hans J. Vogel
15 Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging Shibdas Banerjee and Soumen Kanti Manna
16 Compositional Analysis of the Human Microbiome in Cancer Research
Elisa Morales, Jun Chen, and K. Leigh Greathouse
17 Assessing Metabolic Dysregulation in Muscle During Cachexia
Myriam Y Hsu, Paolo E Porporato, and Elisabeth Wyart
18 Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells
Jing Zhang and Qing Zhang
19 Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging
Thomas S. Blacker, Michael D.E. Sewell, Gyorgy Szabadkai, and Michael R. Duchen
20 Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods
Amanda J. Pearson and Elyssia S. Gallagher
21 Hyperpolarized MRI for Studying Tumor Metabolism
Mikko I. Kettunen
22 Overview of Glutamine-Dependency and Metabolic Rescue Protocols
Shuo Qie, Dan He, and Nianli Sang
23 Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype
Andy Patt, Jalal Siddiqui, Bofei Zhang, and Ewy Mathé
24 Bic
