Part I: Manipulation and Modification of Immune Cells: Dendritic Cells
1. Single Step Antigen Loading and Maturation of Dendritic Cells Through mRNA Electroporation of a Tumor-Associated Antigen and a TriMix of Costimulatory Molecules
Daphné Benteyn, An M.T. Van Nuffel, Sofie Wilgenhof, and Aude Bonehill
2. Generation of Multiple Peptide Cocktail-Pulsed Dendritic Cells as a Cancer Vaccine
Hyun-Ju Lee, Nu-Ri Choi, Manh-Cuong Vo, My-Dung Hoang, Youn-Kyung Lee, and Je-Jung Lee
3. Pulsing Dendritic Cells with Whole Tumor Cell Lysates
Laura Alaniz, Manglio M. Rizzo, and Guillermo Mazzolini
4. Antigen Trapping by Dendritic Cells for Anti-Tumor Therapy
Chiranjib Pal
5. Ex Vivo Loading of Autologous Dendritic Cells with Tumor Antigens
Manglio M. Rizzo, Laura Alaniz, and Guillermo Mazzolini
6. Tumor Antigen/Cytokine-Pulsed Dendritic Cells in Therapy Against Lymphoma
Sumit K. Hira, Deepak Verma, and Partha P. Manna
7. Dendritic Cells Primed with Protein-Protein Fusion Adjuvant
Liying Wang and Yongli Yu
8. Antigen-Specific mRNA Transfection of Autologous Dendritic Cells
Fabian Benencia
9. Electroporation of Dendritic Cells with Autologous Total RNA from Tumor Material
Francesca Milano and K.K. Krishnadath
10. Dendritic Cells Transfected with Adenoviral Vectors as Vaccines
Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, and James E. Talmadge
11. Genetic Modification of Dendritic Cells with RNAi
Xiao-Tong Song
12. Fast Monocyte-Derived Dendritic Cells-Based Immunotherapy
Gamal Ramadan
13. Intratumoral Injection of BCG-CWS Pretreated Dendritic Cells Following Tumor Cryoablation
Naoshi Kawamura, Masaru Udagawa, Tomonobu Fujita, Toshiharu Sakurai, Tomonori Yaguchi, and Yutaka Kawakami
14. Exploiting the CD1d-iNKT Cell Axis for Potentiation of DC-Based Cancer Vaccines
Roeland Lameris, Famke L. Schneiders, Tanja D. de Gruijl, and Hans J. van der Vliet
Part II: Manipulation and Modification of Immune Cells: T Lymphocytes and NK Cells
15. Modification of T Lymphocytes to Express Tumor Antigens
Aaron E. Foster and Xiao-Tong Song
16. Genetic Modification of Mouse Effector and Helper T Lymphocytes Expressing a Chimeric Antigen Receptor
Liza B. John, Tess M. Chee, David E. Gilham, and Phillip K. Darcy
17. Genetic Modification of Cytotoxic T Lymphocytes to Express Cytokine Receptors
Serena K. Perna, Barbara Savoldo, and Gianpietro Dotti
18. Monitoring the Frequency and Function of Regulatory T Cells and Summary of the Approaches Currently Used to Inhibit Regulatory T Cells in Cancer Patients
Chiara Camisaschi, Marcella Tazzari, Licia Rivoltini, and Chiara Castelli
19. Cytokine Activation of Natural Killer Cells
Syh-Jae Lin, Pei-Tzu Lee, and Ming-Ling Kuo
Part III: Manipulation and Modification of Tumor Cells
20. Loading of Acute Myeloid Leukemia Cells with Poly(I: C) by Electroporation
Eva Lion, Charlotte M. de Winde, Viggo F.I. Van Tendeloo, and Evelien L.J.M. Smits
21. Autologous Tumor Cells Engineered to Express Bacterial Antigens
Vijayakumar K. Ramiya, Maya M. Jerald, Patricia D. Lawman, and Michael J.P. Lawman
22. Tumor Cell Transformation Using Antisense Oligonucleotide
Mohamed R. Akl and Nehad M. Ayoub
23. The Direct Display of Costimulatory Proteins on Tumor Cells as a Means of Vaccination for Cancer Immunotherapy
Haval Shirwan, Esma S. Yolcu, Rajesh K. Sharma, Hong Zaho, and Orlando Grimany-Nuno
Part IV: Manipulation of Immune/Tumor Interactions
24. Cloning Variable
