Cardiovascular Development

Preface

Contributors

Part I: Model organisms

1. Use of Whole Embryo Culture for Studying Heart Development

Calvin T. Hang, and Ching-Pin Chang

2. Quantifying Cardiac Functions in Embryonic and Adult Zebrafish

Tiffany Hoage, Yonghe Ding, and Xiaolei Xu

3. Analysis of the Patterning of Cardiac Outflow Tract and Great Arteries with Angiography and Vascular Casting

Ching-Pin Chang

4. Morpholino Injection in Xenopus

Panna Tandon, Chris Showell, Kathleen Christine, and Frank L Conlon

5. Chicken Chorioallantoic Membrane Angiogenesis Model

Domenico Ribatti

6. Visualizing Vascular Networks in Zebrafish: An Introduction to Microangiography

Christopher E. Schmitt, Melinda B. Holland, and Suk-Won Jin

7. Whole-mount Confocal Microscopy for Vascular Branching Morphogenesis

Yoh-suke Mukouyama, Jennifer James, Joseph Nam, and Yutaka Uchida

8. Visualization of Mouse Embryo Angiogenesis by Fluorescence-based Staining

Yang Liu, Marc Antoniyak, and Xu Peng

9. Miniaturized Assays of Angiogenesis in Vitro

May J. Reed and Robert B. Vernon

Part II: Cell and Molecular Biology Methods

10. Analysis of the Endocardial-to-Mesenchymal Transformation of Heart Valve Development by a Collagen Gel Culture Assay

Ching-Pin Chang, Yiqin Xiong, and Bin Zhou.

11. Quantification of Myocyte Chemotaxis: A role for FAK in Regulating Directional Motility

Britni Zajac, Zeenat S. Hakim, Morgan V. Cameron, Oliver Smithies, and Joan M. Taylor

12. Analysis of Neural Crest Cell Fate During Cardiovascular Development using Cre-activated lacZ/b-galactosidase Staining

Yanping Zhang and L. Bruno Ruest

13. Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte

Jieli Li, Marc Antonyak, and Xu Peng

14. Isolation and Characterization of Vascular Endothelial Cells from Murine Heart and Lung

Yixin Jin, Yang Liu, Marc Antonyak, and Xu Peng

15. Isolation and Characterization of Embryonic and Adult Epicardium and Epicardium-Derived Cells

Bin Zhou and William T. Pu

16. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization

Jan Patterson, Rick Metz, and Emily Wilson

17. C-kit Expression Identifies Cardiac Precursor Cells

Michael Craven, Michael I. Kotlikoff, and Alyson S. Nadworny

Dongfei Qi and Mingui Fu

19. Adenovirus-mediated Gene Transfection in the Isolated Lymphatic Vessels

Anatoliy A. Gashev, Mariappan Muthuchamy, and David C. Zawieja

20. Isolation of Cardiac Myocytes and Fibroblasts from Neonatal Rat Pups

Honey B. Golden, Deepika Gollapudi, Fnu Gerilechaogetu, Jieli Li, Ricardo J. Cristales,

Xu Peng, and David E. Dostal

Part III: New Techniques

21. The Application of Genome-wide RNAi Screens in Exploring Varies of Signaling Transduction Pathways

Shenyuan Zhang and Hongying Zheng

22. Application of Atomic Force Microscopy Measurements on Cardiovascular Cells

Xin Wu, Zhe Sun, Gerald A. Meininger, and Mariappan Muthuchamy

23. In Utero Assessment of Cardiovascular Function in the Embryonic Mouse Heart using High Resolution Ultrasound Biomicroscopy

Honey B. Golden,