Part I: Caspases
1. General in vitro Caspase Assay Procedures
Dave Boucher, Catherine Duclos, Jean-Bernard Denault
2. Positional Scanning Substrate Combinatorial Library (PS-SCL) Approach to Define Caspase Substrate Specificity
Marcin Poręba, Aleksandra Stróżyk, Paulina Kasperkiewicz, Marcin Drąg
3. Global Identification of Caspase Substrates using PROTOMAP (PROtein TOpography and Migration Analysis Platform)
Melissa M. Dix, Gabriel M. Simon, Benjamin F. Cravatt
4. Caspase-2 Protocols Loretta Dorstyn and Sharad Kumar
5. Caspase-14 Protocols
Mami Yamamoto-Tanaka and Toshihiko Hibino
6. Caspase Protocols in Caenorhabditis elegans
Eui Seung Lee & Ding Xue
7. Detecting Caspase Activity in Drosophila Larval Imaginal Discs
Caitlin E. Fogarty and Andreas Bergmann
8. Methods for the Study of Caspase Activation in the Xenopus laevis Oocyte and Egg Extract
Francis McCoy, Rashid Darbandi, and Leta K. Nutt
9. Caspase Protocols in Mice
Varsha Kaushal, Christian Herzog, Randy S. Haun, and Gur P. Kaushal
10. Measurement of Caspase Activation in Mammalian Cell Cultures
Magnus Olsson and Boris Zhivotovsky
Part II: Paracaspases and Metacaspases
11. Detection and Measurement of Paracaspase MALT1 Activity
Stephan Hailfinger, Christiane Pelzer, and Margot Thome
12. Leishmania Metacaspase: an Arginine-Specific Peptidase
Ricardo Martin, Iveth Gonzalez and Nicolas Fasel
13. Purification, Characterization, and Crystallisation of Trypanosoma Metacaspases
Karen McLuskey, Catherine X. Moss, and Jeremy C. Mottram
14. Monitoring the Proteostasis Function of the Saccharomyces cerevisiae Metacaspase Yca1
Amit Shrestha, Robin E. C. Lee, and Lynn A. Megeney
15. Plant Metacaspase Activation and Activity
Elena A. Minina, Simon Stael, Frank Van Breusegem, and Peter V. Bozhkov
16. Preparation of Arabidopsis thaliana Seedling Proteomes for Identifying Metacaspase Substrates by N-terminal COFRADIC
Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, and Kris Gevaert
