1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolae
Wayne Johnston and Kirill Alexandrov
2. Bioinformatics Analysis and Optimization of Cell-Free Protein Expression
Alexander A. Tokmakova, Atsushi Kurotania, Mikako Shirouzu, Yasuo Fukamid, and Shigeyuki Yokoyama
3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein Expression
Andrew Kralicek
4. One-Pot, Microscale Cell-Free Enzyme Expression and Screening
Aarthi Chandrasekaran and Anup K. Singh
5. Cell-Free Translation of Biofuels Enzymes
Taichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen, and Brian G. Fox
6. Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis Systems
Yong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim
7. High Level Cell-Free Production of Membrane Proteins with Nanodiscs
Christian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma, Slawomir Filipek, Xiaoning Wang, Volker Dötsch, and Frank Bernhard
8. Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction Study
Sabrina Guillemer, Cécile Persillon, Jean-Michel Masson, and Gilles Ravot
9. Human Cell Extract-Derived Cell-Free Systems for Virus Synthesis
Tominari Kobayashi, Kodai Machida, and Hiroaki Imataka
10. Cell-Free Protein Synthesis in Microfluidic 96-Well Plates
Kirsten Jackson, Ruba Khnouf, and Z. Hugh Fan
11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein Synthesis
Kiyoshi Ozawa and Ruhu Qi
12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein Synthesis
Kiyoshi Ozawa and Choy Theng Loh
13. In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free Lysate
Kong-Nan Zhao
14. An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNA
Xiao Wang, Liang Zhao, and Kong-Nan Zhao
15. In vitro Translation Based Protein Kinase Substrate Identification
Szilvia K. Nagy and Tamás Mészáros
16. Preparation of Protein Arrays using Cell-Free Protein Expression
Elizabeth A. Cook and Mingyue He
17. Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free System
Motoaki Wakiyama and Shigeyuki Yokoyama
18. Cell-Free Membrane Protein Expression
Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama
19. The PURE System for Protein Production
Yoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda
20. A Cell-Free Protein Synthesis System from Insect Cells
Toru Ezure, Takashi Suzuki, and Eiji Ando
21. A Cell-Free Expression Platform for Production of Protein Microarrays
Xristo Zárate and David W. Galbraith
