1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing
Agnelo Furtado
2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)
Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa
3. RNA Extraction from Cereal Vegetative Tissue
Julie A. Pattemore
4. RNA Extraction from Developing or Mature Wheat Seeds
Agnelo Furtado
5. cDNA Library Preparation
Maarten Kooiker and Gang-Ping Xue
6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants
Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining
7. The Polymerase Chain Reaction (PCR) - General Methods
Daniel L. E. Waters and Frances M. Shapter
8. Mutation and Mutation Screening
L. Slade Lee, Bradley J. Till, Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak
9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression
Timothy L. Fitzgerald and Richard B. McQualter
10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
Agnelo Furtado
11. Rapid Cloning of Genes and Promoters for Functional Analyses
Peer M. Schenk
12. Genome Walking
Frances M. Shapter and Daniel L.E. Waters
13. Functional Analysis by Protein Biochemistry
Louis M. T. Bradbury
14. Genomic Southern Blot Analysis
Leigh Gebbie
15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs
Shazia Iram
16. Protein Blotting-Protocol for Beginners
Lars A. Petrasovits
17. Genetic Transformation of Wheat via Particle Bombardment
Caroline A. Sparks & Huw D. Jones
18. Sorghum Genetic Transformation by Particle Bombardment
Guoquan Liu, Bradley C. Campbell and Ian D. Godwin
19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery
Caroline A. Sparks, Angela Doherty & Huw D. Jones
20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation
Wendy A Harwood
21. Agrobacterium-mediated Transformation- Rice Transformation
Inez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo
