Part I. Chemical Probes of Membrane Protein Structure and Function
1. Engineering K+ Channels using Semisynthesis
Alexander G. Komarov, Corey A. Costantino, and Francis I. Valiyaveetil
2. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes
Zhengmao Hua and William R. Kobertz
3. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules
Sviatoslav N. Bagriantsev and Daniel L. Minor Jr.
4. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers
Jessica H. Harvey, Richard M. van Rijn, and Jennifer L. Whistler
Part II. Photochemical Control of Protein and Cellular Function
5. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy
Joseph P. Y. Kao and Sukumaran Muralidharan
6. Characterization of One- and Two- Photon Photochemical Uncaging Efficiency
Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, and Maurice Goeldner
7. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization
Alexandre Mourot, Timm Fehrentz, and Richard H. Kramer
8. A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable Ligands
Matthew R. Banghart and Dirk Trauner
9. Developing a Photoreactive Antagonist
Pamela M. England
Part III. Chemical Probes for Imaging in the Nervous System
10. Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors
Laurel A. Cooley, Vladimir V. Martin, and Kyle R. Gee
11. Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation
Corey D. Acker and Leslie M. Loew
12. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor
Ruud Hovius
13. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots
Qi Zhang
14. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI
Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, and Alan Jasanoff
