1. A Surgical Approach to Hindlimb Suspension: A Mouse Model of Disuse-Induced Atrophy
Laura Kutz, Tong Zhou, Qi Chen, and Hua Zhu
2. Remote Ischemic Pre-Conditioning (RIPC)
Kyung Eun Lee, Jongsoo Kim, Ki Ho Park, Jianjie Ma, and Hua Zhu
3. Mouse Corneal Transplantation
Peng Chen, Ki Ho Park, Liqiang Zhang, Alexandra R. Lucas, Heather L. Chandler, and Hua Zhu
4. Analysis of Chemokine-to-GAG Interactions in Model of Donor Renal Allograft Transplant
Yanlin Ma and Hao Chen
5. Mouse Models of Renal Allograft Transplant Rejection: Methods to Investigate Chemokine: GAG Interaction and Therapeutic Blockade
Isabela R. Zanetti, Liqiang Zhang, Michelle Burgin, Jacquelyn Kilbourne, Jordan R. Yaron, David Fonseca, and Alexandra R. Lucas
6. Ex Vivo Perfusion System to Analyze Chemokine-Driven Leukocyte Adhesion
Sanne L. Maas, Remco T.A. Megens, and Emiel P.C. van der Vorst
7. In Vitro (Trans)migration Experiment Using Chemokines as Stimulatory Factor
Sanne L. Maas and Emiel P.C. van der Vorst
8. The Use of Campenot Trichambers for the Study of Peripheral Neuronal Growth and Survival in Presence of Thrombotic Factors and Serpins
Wesley M. Tierney and Ian B. Hogue
9. Flow Cytometry Analysis of Immune Cell Responses
Kavita R. Manhas and Joseph N. Blattman
10. Detection of Chemokine Binding Proteins Association to Cell Surface Glycosaminoglycans by Flow Cell Cytometry and Indirect Immunofluorescence
Bruno Hernaez and Antonio Alcamí
11. Protein Microarrays and Their Fabrication
Mahasish Shome and Joshua LaBaer
12. NMR Methods for Characterization of Glycosaminoglycan-Chemokine Interactions
Vitor H. Pomin and Krishna Rajarathnam
13. Glycosaminoglycan Analysis: Purification, Structural Profiling, and GAG-Protein Interactions
Amrita Basu and Ryan J. Weiss
14. Isolation and Compositional Analysis of Glycosaminoglycans
Stephanie Archer-Hartmann, Lauren E. Pepi, Christian Heiss, and Parastoo Azadi
15. How to Design Peptides
Joseph Dodd-o, Amanda M. Acevedo-Jake, Abdul-Rahman Azizogli, Vikram Khipple Mulligan, and Vivek A. Kumar
16. Methods to Assess Chemokine Binding and Anti-Chemotactic Activity of Virus Proteins
Elham Torbati, Gabriella Stuart, Kurt Krause, Chris Brown, and Lyn Wise
17. Producing Biologics with Defined N-Glycosylation in Plants
