1: Ex-Vivo signal Transduction Studies in Chronic Lymphocytic Leukemia
Karly-Rai Rogers-Broadway, Laura Karydis, Rachel C Dobson, and Andrew J Steele
2: Ex vivo Pharmacological Profiling in Chronic Lymphocytic Leukemia Cells
Burcu Aslan, Mary L. Ayres, and Varsha Gandhi
3: Culture and Harvest of CpG-stimulated Blood and Bone Marrow in Chronic Lymphocytic Leukemia
Cecelia R. Miller and Nyla A. Heerema
4: Analysis of Common Abnormalities seen in Chronic Lymphocytic Leukemia using Fluorescence in situ Hybridization
Reid G. Meyer and Daniel L. Van Dyke
5: Immunoglobulin Gene Analysis in Chronic Lymphocytic Leukemia
Andreas Agathangelidis, Richard Rosenquist, Frederic Davi, Paolo Ghia, Chrysoula Belessi, Anastasia Hadzidimitriou, and Kostas Stamatopoulos
6: Detection and Functional Analysis of TP53 Mutations in CLL
Sarka Pavlova, Jana Smardova, Nikola Tom, and Martin Trbusek
7: The Development and Use of Scalable Systems for Studying Aberrant Splicing in SF3B1-mutant CLL
Tushar Murthy, Kiran V. Paul, Alexander C. Minella, and Manoj M. Pillai
8: Methods for Investigating VLA-4 (CD49d/CD29) Expression and Activation in Chronic Lymphocytic Leukemia and its Clinical Applications
Antonella Zucchetto, Erika Tissino, Alexander Chigaev, Tanja Nicole Hartmann, and Valter Gattei
9: In vitro Assay to Study CLL and Monocyte Interactions
Maria Teresa Sabrina Bertilaccio, Ronghua Zhang, Priyanka Banerjee, and Varsha Gandhi
10: Extracellular Flux Assays to Determine Oxidative Phosphorylation and Glycolysis in Chronic Lymphocytic Leukemia Cells
Hima V. Vangapandu and Varsha Gandhi
11: Measurement of Leukemic B-cell Growth Kinetics in Patients with Chronic Lymphocytic Leukemia
Andrea N. Mazzarello, Mark Fitch, Marc K. Hellerstein, and Nicholas Chiorazzi
12: Assays on DNA Damage and Repair in CLL
Tzung-Huei Lai and Deepa Sampath
13: Method for Generating a Patient-Derived Xenograft Model of CLL
Shih-Shih Chen
14: Immunological Methods to Study Monoclonal Antibody Activity in Chronic Lymphocytic Leukemia
MJ Carter, LN Dahal, KLS Cleary, MJE Marshall, RR French, SA Beers, and MS Cragg
15: CRISPR/Cas9-based Gene Dropout Screens
Kai Wu and Sami N. Malek
16: Gene Disruption using CRISPR-Cas9 Technology
Nan Hu and Sami N. Malek
17: Minimal Residual Disease Quantification in Chronic Lymphocytic Leukemia: Clinical Significance and Flow Cytometric Methods
Sebastian Böttcher
18: Rare Variant Quantitation Using Droplet Digital PCR
Brian Parkin
19: Methods for Measuring ctDNA in Lymphomas
Davide Rossi, Adalgisa Condoluci, Valeria Spina, and Gianluca Gaidano
20: Measurement of miRNAs in Chronic Lymphocytic Leukemia Patient Samples by Quantitative Reverse Transcription PCR
Katrien Van Roosbroeck, Recep Bayraktar, and George A. Calin
21: Bioinformatics Analysis of Whole Exome Sequencing Data
Peter J. Ulintz, Weisheng Wu, and Chris M. Gates
22: Genome Sequencing and Analysis Methods in Chronic Lymphocytic Leukemia
Víctor Quesada, Miguel Araujo-Voces, José G. Pérez-Silva, Gloria Velasco, and Carlos López-Otín
