Cis-Thf- Und Cis-Thp-Aminosauren ALS Bausteine Fur Gramicidin a Hybrid-Ionenkanale
Cis-Thf- Und Cis-Thp-Aminosauren ALS Bausteine Fur Gramicidin a Hybrid-Ionenkanale

Cis-Thf- Und Cis-Thp-Aminosauren ALS Bausteine Fur Gramicidin a Hybrid-Ionenkanale


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About the Book

Das Antibiotikum Gramicidin A (gA) ist ein Pentadecapeptid aus alternierenden L- und D-Aminosauren, welches sich beta-helical in eine Membran einbauen kann. Es ist in der Lage monovalente Kationen durch eine Membran zu transportieren. Fruhere Arbeiten auf diesem Gebiet zeigten, dass der Austausch von Aminosauren in der Sequenz des gA zu einer Veranderung der Eigenschaften der Kanalfunktion fuhrt. In dieser Arbeit wurden eine cis-THF-Aminosaure und eine cis-THP-Aminosaure als Bausteine fur die Entwicklung neuer Hybrid-Ionenkanale verwendet. Es wurde der Einfluss dieser beiden Bausteine auf die Struktur untersucht. Dabei konnte festgestellt werden, dass beide Bausteine unterschiedliche Auswirkungen auf die Struktur des Peptides haben. Mittels eines Screenings uber einen Teilbereich der Sequenz des gA, bei dem zwei naturliche Aminosauren gegen die artifiziellen Aminosaurebausteine ausgetauscht wurden, konnte festgestellt werden, dass Position 11-12 der gA-Sequenz die optimale Position fur den Austausch ist. Hier konnte mit beiden Bausteinen die besten Leitfahigkeiten und Selektivitaten gemessen werden.


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Product Details
  • ISBN-13: 9783832512361
  • Publisher: Logos Verlag Berlin
  • Sub Title: Synthese, Struktur- Und Funktionsuntersuchung
  • ISBN-10: 3832512365
  • Publisher Date: 10 Jun 2006


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