1. Purification of Clathrin-coated Vesicles from Adult Rat Brain
Silvia Giovedì
2. Preparation of Synaptosomes from Mammalian Brain by Subcellular Fractionation and Gradient Centrifugation
Mirko Messa
3. Probing Endocytosis during the Cell Cycle with Minimal Experimental Perturbation
António JM Santos and Emmanuel Boucrot
4. Assaying the Contribution of Membrane Tension to Clathrin-mediated Endocytosis
Steeve Boulant
5Identifying Small Molecule Inhibitors of the Clathrin Terminal Domain
Volker Haucke and Michael Krauß
6. Acute Manipulations of Clathrin-mediated Endocytosis at Presynaptic Nerve Terminals
Rylie B. Walsh, Ona E. Bloom, and Jennifer R. Morgan
7. Imaging "Hot-wired" Clathrin-mediated Endocytosis
Laura A. Wood and Stephen J. Royle
8. Real-time Endocytosis Measurements by Membrane Capacitance Recording at Central Nerve Terminals
Xuelin Lou
9. Assaying Mutants of Clathrin Mediated Endocytosis in the Fly Eye
Elsa Lauwers and Patrik Verstreken
10. Reconstitution of Clathrin Coat Disassembly for Fluorescence Microscopy and Single-molecule Analysis
Till Böcking, Srigokul Upadhyayula, Iris Rapoport, Benjamin R. Capraro, and Tomas Kirchhausen
11. Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in Isolated Plasma Membranes
Ira Milosevic
12. SMrT Assay for Real-time Visualization and Analysis of Clathrin Assembly Reactions
Devika Andhare, Sachin S. Holkar, and Thomas J. Pucadyil
13. Real-time Monitoring of Clathrin Assembly Kinetics in a Reconstituted System
Jeffery Yong, Yan Chen, and Min Wu
14. Stimulated Emission Depletion (STED) Imaging of Clathrin-mediated Endocytosis in Living Cells
Francesca Bottanelli and Lena Schroeder
15. Measuring Clathrin-coated Vesicle Formation with Single-molecule Resolution
François Aguet and Emanuele Cocucci
16. Cryo-electron Tomography of the Mammalian Synapse
Rubén Fernández-Busnadiego
17. Quantitative Analysis of Clathrin-mediated Endocytosis in Yeast by Live Cell Fluorescence Microscopy
Eric B Lewellyn and Yansong Miao
18. Using FM Dyes to Monitor Clathrin-mediated Endocytosis in Primary Neuronal Culture
Michael A. Cousin, Sarah L. Gordon, and Karen J. Smillie