Part I: Clinical Metabolomics and Lipidomics

1. Metabolomics as a Tool to Understand Pathophysiological Processes

Julijana Ivanisevic and Aurelien Thomas

2. Metabolomics in Immunology Research

Bart Everts

Part II: LC-MS-Based Metabolomics

3. LC-MS-Based Metabolomics of Biofluids Using All Ion Fragmentation (AIF) Acquisition

4. Lipid Mediator Metabolomics via LC-MS/MS Profiling and Analysis

Jesmond Dalli, Romain A. Colas, Mary E. Walker, and Charles N. Serhan

5. UHPSFC/ESI-MS Analysis of Lipids

Miroslav Lísa and Michal Holčapek

6. LC-MS/MS Analysis of Lipid Oxidation Products in Blood and Tissue Samples

Yiu Yiu Lee and Jetty Chung-Yung Lee

7. Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum

8. LC-MS/MS Analysis of Bile Acids

Sabrina Krautbauer and Gerhard Liebisch

9. LC-MS/MS Analysis of Triglycerides in Blood-Derived Samples

Madlen Sander, Susen Becker, and Uta Ceglarek

10. LC-MS/MS Analysis of the Epoxides and Diols Derived from the Endocannabinoid Arachidonoyl Ethanolamide

11. Sphingolipid Analysis in Clinical Research

Bo Burla, Sneha Muralidharan, Markus R. Wenk, and Federico Torta

12. Shotgun Lipidomics Approach for Clinical Samples

Lars F. Eggers and Dominik Schwudke

13. Establishing and Performing Targeted Multi-Residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples

Theresa L. Pedersen and John W. Newman

14. Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis

Wei Han and Liang Li

15. Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma & Serum Using the Lipidyzer^TM Platform

Baljit K. Ubhi

Part III: GC-MS-Based Metabolomics

16. Exploratory GC-MS-Based Metabolomics of Body Fluids

Carole Migné, Stéphanie Durand, and Estelle Pujos-Guillot

17. GC-MS Analysis of Short-Chain Fatty Acids in Feces, Cecum Content, and Blood-Derived Samples

18. GC-MS Analysis of Medium- and Long-Chain Fatty Acids in Blood Samples

Lisa Hoving, Marieke Heijink, Vanessa van Harmelen, Ko Willems van Dijk, and Martin Giera

19. Analysis of Oxysterols

Fabien Riols and Justine Bertrand-Michel

20. Analysis of Metabolites from the Tricarboxylic Acid Cycle for Yeast and Bacteria Samples Using Gas Chromatography Mass Spectrometry

Reza Maleki Seifar, Angela ten Pierick, and Patricia T.N. van Dam

21. GC-MS Analysis of Lipid Oxidation Products in Blood, Urine, and Tissue Samples

Anne Barden and Trevor A. Mori

Part IV: CE-MS-Based Metabolomics

22. Metabolic Profiling of Urine by Capillary Electrophoresis-Mass Spectrometry Using Non-Covalently Coated Capillaries

Rawi Ramautar

23. CE-MS for the Analysis of Amino Acids

Karina Trevisan Rodrigues, Marina Franco Maggi Tavares, and Ann Van Schepdael

Part V: NMR-Based Metabolomics