1. Advances in Confocal Microscopy and Selected Applications
W. Matt Reilly and Christopher J. Obara
2. Choosing Fluorescent Probes and Labeling Systems
Jacoby Morris and George H. Patterson
3. General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy
Joseph A. Brzostowski
4. Microfabricated devices for confocal microscopy on biological samples
Nicole Y. Morgan
5. Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging
Xufeng Wu and John A. Hammer
6. High-resolution multicolor imaging of mitochondria in lymphocytes
Munir Akkaya, Pietro Miozzo, and Margery G. Smelkinson
7. Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy Paul Tillberg
8. Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy Abhijit Ashok Ambegaonkar and Haewon Sohn
9. Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight Sara Hernández-Pérez, Marika Vainio, Vid Sustar, and Pieta K. Mattila
10. Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum Xuehua Xu, Joseph Brzostowski, Sharmila Ramachandra, Smit Bhimani, Yan You, and Tian Jin
11. Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis
Miao Pan and Tian Jin
12. High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains
Mojca Mattiazzi Usaj, Dara S Lo, Ben T Grys, and Brenda J Andrews
13. Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy
Billur Akkaya, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger
14. Studying Neuronal Biology Using Spinning Disc Confocal Microscopy
Javier Manzella-Lapeira, Joseph Brzostowski, and Jenny Serra-Vinardell
15. Method for Acute Intravital Imaging of the Large Intestine in Live Mice
Erreni Marco, Doni Andrea and Roberto Weigert
16. Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism
Javier Manzella-Lapeira and Joseph Brzostowski
17. Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry
Greg Alspaugh, Branden Roarke, Alexandra Chand, Rozhin Penjweini, Alessio Andreoni, and Jay R. Knutson
18. FLIM Imaging for Metabolic Studies in Live Cells