Crispr Gene Editing Book by Yonglun Luo - Bookswagon
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Crispr Gene Editing

Crispr Gene Editing


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About the Book

Part I: Methods for CRISPR-gRNA Design and Quantification of Activity

1. CRISPR-gRNA Design

Maria Pallarès Masmitjà, Nastassia Johanna Knödlseder, and Marc Güell

2. Tracking CRISPR's Footprints

Lin Lin and Yonglun Luo

3. Rapid Quantitative Evaluation of CRISPR Genome Editing by TIDE and TIDER

Eva Karina Brinkman and Bas van Steensel

4. Fast and Quantitative Identification of Ex Vivo Precise Genome Targeting-Induced Indel Events by IDAA

Saskia König, Yang Zhang, Hans Heugh Wandall, Claudio Mussolino, and Eric Paul Bennett

5. Functional Evaluation of CRISPR Activity by the Dual-Fluorescent Surrogate System: C-Check

Lin Lin and Yonglun Luo

Part II: Methods for CRISPR Delivery

6. CRISPR-Cas9 Delivery by Artificial Virus (RRPHC)

Suleixin Yang, Qinjie Wu, Yuquan Wei, and Changyang Gong

7. Production and Validation of Lentiviral Vectors for CRISPR/Cas9 Delivery

Laura Barrett Ryø, Emil Aagaard Thomsen, and Jacob Giehm Mikkelsen

8. Rapid and Simple Screening of CRISPR Guide RNAs (gRNAs) in Cultured Cells Using Adeno-Associated Viral (AAV) Vectors

Julia Fakhiri, Manuela Nickl, and Dirk Grimm

9. Electroporation-Based CRISPR/Cas9 Gene Editing Using Cas9 Protein and Chemically Modified sgRNAs

Anders Laustsen and Rasmus O. Bak

Part III: CRISPR Gene Editing in Human iPSCs

10. Efficient Gene Editing of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Saniye Yumlu, Sanum Bashir, Jürgen Stumm, and Ralf Kühn

11. Editing the Genome of Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 Ribonucleoprotein Complexes

Michaela Bruntraeger, Meg Byrne, Kathleen Long, and Andrew R. Bassett

12. Conditional Gene Knockout in Human Cells with Inducible CRISPR/Cas9

Kirsten E. Snijders, James D. Cooper, Ludovic Vallier, and Alessandro Bertero

Part IV: CRISPR Gene Editing in Other Cell Types

13. CRISPR/Cas9 as a Genome Editing Tool for Targeted Gene Integration in CHO Cells

Daria Sergeeva, Jose Manuel Camacho-Zaragoza, Jae Seong Lee, and Helene Faustrup Kildegaard

14. Rapid and Efficient Gene Deletion by CRISPR/Cas9

Signe Neldeborg, Lin Lin, Magnus Stougaard, and Yonglun Luo

15. Genome Editing in Mice

Lisbeth Ahm Hansen and Ernst-Martin Füchtbauer

16. CRISPR/Cas9-Mediated Gene Tagging: A Step-by-Step Proto

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Product Details
  • ISBN-13: 9781493991693
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Height: 254 mm
  • No of Pages: 362
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm
  • ISBN-10: 1493991698
  • Publisher Date: 26 Mar 2019
  • Binding: Hardback
  • Language: English
  • Returnable: Y
  • Spine Width: 22 mm
  • Weight: 920 gr

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