PART I: Fundamental Aspects And Freezing Technology
1 Principles of Cryopreservation
David E. Pegg
2 Principles of Cryopreservation By Vitrification Gregory M. Fahy and Brian Wowk
3 Modeling and Optimization of Cryopreservation James Benson
4 The Principles of Freeze-Drying Gerald D.J. Adams, Isobel Cook, and Kevin R. Ward
PART II: Methods To Study Freezing and Drying Processes
5. Use of In Situ Fourier Transform Infrared Spectroscopy to Study Freezing and Drying of Cells Willem F. Wolkers and Harriëtte Oldenhof
6. Calorimetric Analysis Of Cryopreservation and Freeze-Drying Formulations
Wendell Q. Sun
7. Measurement Of Intracellular Ice Formation Kinetics By High-Speed Video Cryomicroscopy
Jens O. M. Karlsson
8. Laser Scanning Microscopy in Cryobiology Frank Stracke, Asger Kreiner-Møller, and Heiko Zimmermann
9. Low Temperature Electron Microscopy Roland A. Fleck
PART III: Cryopreservation Protocols
10 Cryopreservation of Semen From Domestic Livestock Harald Sieme, Harriëtte Oldenhof
11. Cryopreservation of Mammalian Oocytes Victoria Keros, Barry J. Fuller
12. Vitrification: A Simple And Successful Method for Cryostorage of Human Blastocysts
Juergen Liebermann
13 Efficient Cryopreservation of Human Pluripotent Stem Cells By
Surface-Based Vitrification Julia C. Neubauer, Axel F. Beier, Nils Geijsen, and Heiko Zimmermann
14 Cryopreservation Of Greenshell(TM) Mussel (Perna Canaliculus) Sperm Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. Mcgowan, and
H. Robin Tervit
15 Membrane Modification Strategies for Cryopreservation
Phillip H. Purdy and James K. Graham
16. Sperm Clean-Up and Density Centrifugation Procedures
Harald Sieme and Harriëtte Oldenhof
17. Cryopreservation of Red Blood Cells Johan W. Lagerberg
18. Cord Blood Clinical Processing, Cryopreservation, and Storage
Heidi Elmoazzen and Jelena L. Holovati
19. Directional Freezing For Large Volume Cryopreservation Joseph Saragusty
20 Vitrification Of Heart Valve Tissues Kelvin G.M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene,
and Lia H. Campbell
21 Cryopreservation of Plant Cell Lines Heinz Martin Schumacher, Martina Westphal, and Elke Heine-Dobbernack
22 Writing Standard Operating Procedures (Sops)
