About the Book
Preface to the Series
Preface
Table of Contents
Contributing Authors
PART I Introduction
1. Brain Proteomics: Decoding Neuroproteomes using Mass-Spectrometry
Joaquín Fernández-Irigoyen and Enrique Santamaría
PART II Labeling Methods in Neuroproteomics
2. Applications of Amine-Reactive Tandem Mass Tags (TMT) in Human Neuroproteomics
Linnéa Lagerstedt, Leire Azurmendi, and Jean-Charles Sanchez
3. Application of Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) to Monitor Olfactory Proteomes during Alzheimer´s Disease Progression
Andrea González-Morales, Mercedes Lachén-Montes, María Ibañez-Vea, Enrique Santamaría, and Joaquín Fernández-Irigoyen
4. Protein Microarrays in Neurodegenerative Diseases
Pablo San Segundo-Acosta, María Garranzo-Asensio, Ana Montero-Calle, Carmen Oeo-Santos, Mayte Villalba, Ana Guzmán-Aránguez, and Rodrigo Barderas
PART III Label-Free Methods in Neuroproteomics5. Comprehensive Shotgun Proteomic Analyses of Oligodendrocytes using Ion Mobility and Data Independent Acquisition
Juliana S. Cassoli and Daniel Martins-de-Souza
6. Non-Targeted Brain Lipidomics Profiling Performed by UPLC-ESI-qTOF-MS/MS
Alba Naudí, Rosanna Cabré, Mariona Jové, and Reinald Pamplona
7. Methods for Human Olfactory Bulb-Tissue Studies using Peptide/Protein MALDI-TOF Imaging Mass Spectrometry (MALDI-IMS)
Ibon Iloro, Joaquín Fernández-Irigoyen, Iraide Escobes, Mikel Azkargorta, Enrique Santamaría, Felix Elortza
8. Neuroproteomics using Short GeLC-SWATH: From the Evaluation of Proteome Changes to the Clarification of Protein Function
Sandra I. Anjo, Cátia Santa, Susana C. Saraiva, Karolina Freitas, Faraj Barah, Bruno Carreira, Inês Araújo, and Bruno Manadas
PART IV Mass Spectrometry-based Neuroproteomics to Analyze Post-Translational Modificiations
9. Analysis of Brain Phosphoproteome using Titanium Dioxide Enrichment and High Resolution LC-MS/MS
Jeffrey M. Sifford, Haiyan Tan, Hong Wang, and Junmin Peng
10. N-Glycomics and N-Glycoproteomics of Human Cerebrospinal Fluid
Sophie Cholet, Arnaud Goyallon, Christophe Junot, and François Fenaille
11. In Vivo Strategies to Isolate and Characterize the Neuronal Ubiquitinated Proteome
Juanma Ramírez, Nagore Elu, Aitor Martinez, Benoit Lectez, and Ugo Mayor
12. Characterization of the Phosphoproteome and Sialoproteome in Brain Tissues by Mass Spectrometry
María Ibañez-Vea, Stefan J. Kempf, and Martin R. Larsen
13. Proteomic Analysis of SUMOylation in the Post-Ischemic Brain
J. Will Thompson, Meng Jiang, and Wei Yang
14. S-Nitrosylation in Alzheimer´s Disease using Oxidized Cysteine-Selective cPILOT
Ryan R. Dyer, Liqing Gu, and Renã A.S. Robinson
PART V Subcellular Neuroproteomics
15. Proteomic Analysis of Extracellular Vesicles in Neurological Diseases
Matías Sáenz-Cuesta, Enrique Santamaría, Joaquín Fernández-Irigoyen, and David Otaegui
16. Quantitative In-Depth Profiling of the Postsynaptic Density Proteome to Understand the Molecular Mechanisms Governing Synaptic Physiology and Pathology
Rita Reig-Viader and Alex Bayés
17. Nuclear Proteomics for Exploring MK-801 Treated Oligodendrocytes to Better Understand Schizophrenia
Aline G. Santana, Giuliana S. Zuccoli, Verônica Saia-Cereda, Juliana S. Cassoli, and Daniel Martins-de-Souza
18. Localized Proteomics of Individual Neurons Isolated from Formalin-Fixed, Para
