1: Molecular Methods for the Study of Signal Transduction in Plants
Helen R. Irving and Chris Gehring
2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains
Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi
3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS
Heike Burhenne and Volkhard Kaever
4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts
Richard M. Graeff and Hon Cheung Lee
5: In vivo Imaging of cGMP in Plants
Jean-Charles Isner and Frans J.M. Maathuis
6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses
Fouad Lemtiri-Chlieh and Rashid Ali
7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides
Natalia Maria Ordoñez, Lana Shabala, Chris Gehring, and Sergey Shabala
8: Calcium Imaging of the Cyclic Nucleotide Response
Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya
9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation
Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze
10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules
Claudius Marondedze, Kathryn Lilley and Ludivine Thomas
11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome
Lara Donaldson and Stuart Meier
12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity
Victor Muleya, Janet I. Wheeler and Helen R. Irving
13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants
Aloysius Wong and Chris Gehring
14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions
Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz
15: Inferring biological functions of guanylyl cyclases with computational methods
May Alqurashi and Stuart Meier
16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases
Erin B. Purcell and Rita Tamayo
17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants
Robin K. Walker and Gerald A. Berkowitz
18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin
Ndiko Ludidi
19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses
Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito