Part I: Pathophysiology of Cystic Fibrosis
1. Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology
Mark T. Clunes and Richard C. Boucher
2. Imaging CFTR Protein Localization in Cultured Cells and Tissues
Silvia M. Kreda and Martina Gentzsch
3. CFTR Regulation of Epithelial Sodium Channel
Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, and Bakhrom K. Berdiev
4. Methods for Evaluating Inflammation in Cystic Fibrosis
Assem G. Ziady and Pamela B. Davis
5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures
Erin N. Worthington and Robert Tarran
6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands
Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyungju Cho, Mauri E. Krouse, Jin V. Wu, Monal Khansaheb, Toshiya Irokawa, Juan Ianowski, John W. Hanrahan, Alan W. Cuthbert, and Kim V. Tran
7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia
Carla M.P. Ribeiro
8. Identification and Quantification of Mucin Expression
Kristina A. Thomsson and Gunnar C. Hansson
9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis
Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena Nørgaard, and Niels Høiby
10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells
Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, and Marc Chanson
Part II: Omic Approaches to Study Cystic Fibrosis
11. Introduction to Part II: Omics in the Biology of Cystic Fibrosis
William E. Balch
12. Microarray mRNA Expression Profiling to Study Cystic Fibrosis
Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray, Jr.
13. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)
Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, and Aleksander Edelman
14. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis
William E. Balch and John R. Yates III
15. Functional Genomics Assays to Study CFTR Traffic and ENaC Function
Joana Almaça, Shehrazade Dahimène, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, and Margarida D. Amaral
16. New Lipidomic Approaches in Cystic Fibrosis
Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, and Aleksander Edelman
Part III: Resources
17. Introduction to Part III: Resources for CFTR Research
Margarida D. Amaral
18. Primary Epithelial Cell Models for Cystic Fibrosis Research
Scott H. Randell, M. Leslie Fulcher, Wanda O'Neal, and John C. Olsen
19. Comparative Biology of Cystic Fibrosis Animal Models
John T. Fisher, Yulong Zhang, and John F. Engelhardt
20. CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction
Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino, Christopher M.
