Cytokine Bioassays

Part I: Detection of Released Cytokines by Immunoassays

1. The Challenge of Measuring Elusive Immune Markers by Enzyme Linked Immuno-Spot (ELISPOT) Technique

Maria Faresjö

1. Analysis of the Released Nuclear Cytokine HMGB1 in Human Serum

Haichao Wang, Lin Zhao, Jianhua Li, Shu Zhu, and Meggie Yeung

1. αELISA Based Assay for IP-10 Detection from Filter Paper Samples

Camilla Heldbjerg Drabe, Thomas Blauenfeldt, and Morten Ruhwald

1. Enhanced ELISA Based on Carboxymethylated Dextran Coatings

Benoît Liberelle, Charles Fortier, and Gregory De Crescenzo

1. A Novel Three-Dimensional Biosensor Based on Aluminum Oxide: Application for Early Stage Detection of Human Interleukin-10

Michael Lee, Abdoullatif Baraket, Nadia Zine, Miguel Zabala, Francesca Campabadal, Raffaele Caruso, Maria Giovanna Trivella, Nicole Jaffrezic-Renault, and Abdelhamid Errachid

6. Quantification of Multiple Cytokines and Chemokines Using Cytometric Bead Arrays

Gemma Moncunill, Joseph J. Campo, and Carlota Dobaño

7. A Useful Guide for Analysis of Immune Markers by Fluorochrome (Luminex) Technique

Maria Faresjö

Part II: Cytokine Bioassays

8. Control of Pro-inflammatory Cytokine Release from Human Monocytes with the Use of an Interleukin-10 Monoclonal Antibody

Hardik Patel and Dennis Davidson

9. Cytokine-Induced Neutrophil Chemotaxis Assay

Dennis Davidson and Hardik Patel

10. An In Vitro One-Dimensional Assay to Study Growth Factor-Regulated Tumor Cell-Macrophage Interaction

Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, and Robert J. Eddy

11. Chemotactic Responses by Macrophages to a Directional Source of a Cytokine Delivered by a Micropipette

Michael Cammer and Dianne Cox

12. Assessment of Phagocytic Activity of Cultured Macrophages Using Fluorescence Microscopy and Flow Cytometry

Lokesh Sharma, Wenjun Wu, Sanjay L. Dholakiya, Samir Gorasiya, Jiao Wu, Ravikumar Sitapara, Vivek Patel, Mao Wang, Michelle Zur, Shloka Reddy, Nathan Siegelaub, Katrina Bamba, Frank A. Barile, and Lin L. Mantell

13. Assessment of Cytokine-Modulated Proteasome Activity

Christopher J. Kirk, Saul R. Powell, and Edmund J. Miller

14. Evaluation of the Adverse Effect of Low Concentration of Cadmium on Interleukin-4 Induced Class Switch Recombination in Burkett's Lymphoma Raji Cell Line

15. A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-Stimulated Macrophage Cell Lysates

Veronika Miskolci, Désirée Spiering, Dianne Cox, and Louis Hodgson

16. Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylation Method

Mahmud Arif Pavel, Clarissa Lam, Parul Kashyap, Zahra Salehi-Najafabadi, Gurpreet Singh, and Yong Yu

17. Detection of CXCR2 Cytokine Receptor Surface Expression Using Immunofluorescence

Clarissa Lam, Mahmud Arif Pavel, Parul Kashyap, Zahra Salehi-Najafabadi, Victoria Valentino, and Yong Yu

18. Detecting Tie2, an Endothelial Growth Factor Receptor, by Using Immunohistochemistry in Mouse Lungs

Prajna P. Guha, Sascha A. David, and Chandra C. Ghosh

19. Use of shRNA to Stably Suppress Chemokine Receptor Expression and Function in Human Cancer Cell Lines

Nicole Salazar, Daniel Muñoz, James Hoy, and Bal L. Lokeshwar

Part III. Analysis of Cytokine Intracellular protein levels and Gene Expression

21. Cytokine Detection by Flow Cytometry

Jian-Ge Qiu, Xiao-Long Mei, Zhe-Sheng Chen, and Zhi Shi

22. Analysis of IL-17 Production by Flow Cytometry and ELISPOT Assays

Ling