1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery
Alfons Billiau
2 The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels
Maria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes
3 Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID
Tomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki
4 Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues
Markus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil
5 Evaluating Posttranscriptional Regulation of Cytokine Genes
Bernd Rattenbacher and Paul R. Bohjanen
6 Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay
Latifa Al-Haj and Khalid S.A. Khabar
7 Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo
Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka
8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor
Paulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz
9 Control of the Interferon Response in RNAi Experiments
Jana Nejepinska, Matyas Flemr, and Petr Svoboda
10. shRNA-induced Interferon-Stimulated Gene Analysis
Núria Morral and Scott R. Witting
11 Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
Fabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik
12 Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins
Daniela Novick and Menachem Rubinstein
13 In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells
William C. Adams and Karin Loré
14 A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells
Lennart Zabeau, José Van der Heyden, and Jan Tavernier
