Preface
Acknowledgment
Section 1: Introduction of Acellularization
Section 2: Extra Cellular Matrix (ECM) as a Bioscaffold
Section 3: Methods of Characterization of Acellularized Tissue
§ Histology Staining: H&E, Masson's Trichrome Staining, DAPI Staining
§ Determination amount of Hydroxy lysin and Hydroxy Proline
§ DNA Quantification
§ Scanning Electron Microscopy (SEM)
§ Mechanical Strength (Tensile and Compressive test)
§ Sodium Dodecyl Sulfate (SDS) Detection Method
§ Cytotoxicity Assessment: Live/Dead Assay, MTT Assay
§ Cell Attachment and Cell Proliferation
§ CT Angiography
Section 4: Methods of Sterilization of Acellularized Tissue
Section 5: skin
Chapter 1: Thin Skin
§ History
§ Materials & Methods
§ Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Chapter 2: Thick Skin
§ History
§ Materials & Methods
§ Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Section 6: Cartilage Tissue
Chapter 1: Hyaline cartilage
§ History
§ Materials & Methods § Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Chapter 2: Fibrocartilage
§ History
§ Materials & Methods § Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Chapter 3: Elastic cartilage
§ History
§ Materials & Methods § Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Section 7: Bone Tissue
§ History
§ Materials & Methods § Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Section 8: Cardiovascular System
§ History
§ Materials & Methods § Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Section 9: Digestive System I
Chapter 1: Tongue
§ History
§ Materials & Methods
§ Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Chapter 2: Esophagus
§ History
§ Materials & Methods
§ Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
Chapter 3: Stomach
§ History
§ Materials & Methods
§ Cell seeding
§ Clinical Applications
§ Limitations
§ Conclusion
About the Author:
Dr. Abdol-Mohammad Kajbafzadeh is working as Professor for Tehran University of Medical Sciences, Tehran.
