Part I. Modern Quantitative Virological Methods
1. Dengue Virus Growth, Purification and Fluorescent Labeling
Summer Zhang, Kuan Rong Chan, Hwee Cheng Tan, and Eng Eong Ooi
2. Isolation and Titration of Dengue Viruses by the Mosquito Inoculation Technique
Milly M. Choy and Duane J. Gubler
3. Measuring Antibody Neutralization of Dengue Virus (DENV) Using a Flow Cytometry-Based Technique
A. Ruklanthi de Alwis and Aravinda M. de Silva
4. Dengue Virus Purification and Sample Preparation for Cryo-Electron Microscopy
Joanne L. Tan and Lok Shee Mei
5. Development of a Multiplex Bead-Based Assay to Monitor Dengue Virus Seroconversion
Kaiting Ng and John E. Connolly
6. Pseudo-Infectious Reporter Virus Particles for Measuring Antibody-Mediated
Neutralization and Enhancement of Dengue Virus Infection
Swati Mukherjee, Ted Pierson and Kimberly A. Dowd
7. Cell-Based Flavivirus Infection (CFI) Assay for the Evaluation of Dengue Antiviral Candidates Using High Content Imaging
Kah Hin Tan, Kitti Chan Wing Ki, Satoru Watanabe, Subhash G. Vasudevan and Manoj Krishnan
Part II. Reverse Genetic Systems to Study Virus Replication and Evolution
8. Development and Application of Dengue Virus Reverse Genetic Systems
Andrew Davidson
9. DENV4 Renilla Luciferase Reporter Replicons
Sofia Alcaraz-Estrada, Rosa Del Angel, and R. Padmanabhan
10. Targeted Mutagenesis of Dengue Virus Type 2 replicon RNA by Yeast in vivo
Recombination
Mark Manzano and R. Padmanabhan
11. Identification of Dengue-Specific Human Antibody Fragments Using Phage Display
Moon Y.F. Tay, Chin Chin Lee, Subhash G. Vasudevan and Nicole J. Moreland
12. Next Generation Whole Genome Sequencing of Dengue Virus
Pauline Poh Kim Aw, Paola Florez de Sessions, Andreas Wilm, Long Truong Hoang, Niranjan Nagarajan, October M. Sessions, and Martin Lloyd Hibberd
Part III. Modern Methods to Study Conformation of RNA Proteins and Their Molecular Interactions
13. MPGAfold in Dengue Secondary Structure Prediction
Wojciech K. Kasprzak and Bruce A. Shapiro
14. Insights into Secondary and Tertiary Interactions of Dengue Virus RNA by SHAPE
Joanna Sztuba-Solinska and Stuart F.J. Le Grice
15. Use of Small Angle X-Ray Scattering to Investigate the Structure and Function of Dengue Virus NS3 and NS5
Kyung H. Choi and Marc Morais
16. Identification of Dengue RNA Binding Proteins Using RNA Chromatography and Quantitative Mass Spectrometry
A.M. Ward, J. Gunaratne, M. A. Garcia-Blanco
17. Analysis of Affinity of Dengue Virus Protein Interaction Using BIAcore
Yau Yin Hoe and Susana Geifman Shochat
18. Functional Genomics Approach for the Identification of Human Host Factors Supporting Dengue Viral Propagation
Nicholas J. Barrows, Sharon F. Jamison, Shelton S. Bradrick, Caroline Le Sommer, So Young Kim, James Pearson, and Mariano A. Garcia-Blanco
19. Investigating Dengue Virus Non-Structural Protein 5 (NS5) Nuclear Import
Johanna E. Fraser, Stephen M. Rawlinson, Chunxiao Wang, David A. Jans, and Kylie M. Wagstaff
Part IV. In vitro Enzyme Assays for Antiviral Screening
20. Small Molecule Inhibitor Discovery for Dengue Virus Protease Using High Throughput Screening
Mark Manzano, Janak Padia, and R. Padmanabhan
21. Construction of Dengue Virus Protease Expression Plasmid and in vitro Protease Assay for Screening Antiviral I
