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Difference Gel Electrophoresis (Dige)

Difference Gel Electrophoresis (Dige)


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About the Book

Part I. Fundamentals

1. DIGE: Past and Future

Jonathan S. Minden

2. The Basics of 2D DIGE

Phil Beckett

3. Multifluorescence 2D Gel Imaging and Image Analysis

Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr

4. Assessing Signal-to-Noise in Quantitative Proteomics: Multivariate Statistical Analysis in DIGE Experiments

David B. Friedman

5. Analysis of Proteins Using DIGE and MALDI Mass Spectrometry

Witold M. Winnik, Robert M. DeKroon, Joseph S. Y. Jeong, Mihaela Mocanu, Jennifer B. Robinette, Christina Osorio, Nedyalka N. Dicheva, Eric Hamlett, and Oscar Alzate

6. Synthesis and Validation of Cyanin-Based Dyes for DIGE

Michael E. Jung, Wan-Joong Kim, Nuraly K. Avliyakulov, Merve Oztug, and Michael J. Haykinson

Part II. Methods

7. 2D DIGE Saturation Labeling for Minute Sample Amounts

Georg J. Arnold and Thomas Fröhlich

8. Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE

Hong-Lin Chan, John Sinclair, and John F. Timms

9. Analysis of Protein Post-Translational Modifications Using DIGE-Based Proteomics

Robert M. DeKroon, Jennifer B. Robinette, Cristina Osorio, Joseph S. Y. Jeong, Eric Hamlett, Mihaela Mocanu, and Oscar Alzate

10. Comparative Analyses of Protein Complexes by Blue Native DIGE

Katrin Peters and Hans-Peter Braun

11. 2D DIGE Analysis of Protein Extracts from Muscle Tissue

Cecilia Gelfi and Sara De Palma

12. Combination of Highly Efficient Hexapeptide Ligand Library-Based Sample Preparation with 2D DIGE for the Analysis of the Hidden Human Serum/Plasma Proteome

Sonja Hartwig and Stefan Lehr

13. 2D DIGE Analysis of Serum after Fractionation by ProteoMinerTM Beads

Cynthia Liang, Gek San Tan, and Maxey C. M. Chung

14. Study Design in DIGE-Based Biomarker Discovery

Alexandra Graf and Rudolf Oehler

15. Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery

Megan Penno, Matthias Ernst, and Peter Hoffman

Part III. Applications in Clinical Proteomics

16. Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma

Keun Na, Min-Jung Lee, Hye-Jin Jeong, Hoguen Kim, and Young-Ki Paik

17. 2D DIGE for the Analysis of RAMOS Cells Sub-Proteomes

Marisol Fernández and Juan Pablo Albar

18. Application of Saturation Labeling in Lung Cancer Proteomics

Gereon Poschmann, Barbara Sitek, Bence Sipos, and Kai Stühler

19. Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE

Rommel A. Mathias, Hong Ji, and Richard J. Simpson

20. Method for Protein Subfractionation of Cardiovascular Tissues before DIGE Analysis

Athanasios Didangelos, Xiaoke Yin, and Manuel Mayr

21. Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus (T2DM) Mouse Models

Celia Smith, Davinia Mills, and Rainer Cramer

22. Evaluating the Efficacy of Subcellular Fractionation of Blast Cells Using Live Cell Labeling and 2D DIGE

Yin Ying Ho, Megan Penno, Michelle Perugini, Ian Lewis, and Peter Hoffman

Part IV. Applications in Animal, Plant and Microbial Proteomics

23. DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method

Christina Rode, Traud Winkelmann, Hans-Peter Br

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Product Details
  • ISBN-13: 9781493962051
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Softcover reprint of the original 1st ed. 2012
  • Language: English
  • Returnable: Y
  • Spine Width: 22 mm
  • Weight: 770 gr
  • ISBN-10: 1493962051
  • Publisher Date: 23 Aug 2016
  • Binding: Paperback
  • Height: 254 mm
  • No of Pages: 401
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm

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