Difference Gel Electrophoresis by Kay Ohlendieck (Editor)
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Difference Gel Electrophoresis

Difference Gel Electrophoresis


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About the Book

Part I: Introduction

1. Two Dimensional Gel Electrophoresis and 2D-DIGE

Paula Meleady

Part II: Difference Gel Electrophoresis

2. Comparative DIGE Proteomics

Kay Ohlendieck

3. 2D-DIGE and Fluorescence Image Analysis

Elisa Robotti and Emilio Marengo

4. DIGE Analysis Software and Protein Identification Approaches

Abduladim Hmmier and Paul Dowling

Part III: DIGE Applications

5. Native DIGE: Efficient Tool to Elucidate Protein Interactomes

Diksha Dani and Norbert Dencher

6. Comparative Two-dimensional Fluorescence Gel Electrophoresis

Doreen Ackermann and Simone König

7. DIGE-based Phosphoproteomic Analysis

Taras Stasyk and Lukas Huber

8. DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue

Paul Dowling

9. Comparative 3-sample DIGE Analysis of Skeletal Muscles

Kay Ohlendieck

10. DIGE Analysis of ProteoMiner(TM) Fractionated Serum/Plasma Samples

Sandra Murphy and Paul Dowling

Part IV: Comparative DIGE Tissue Proteomics

11. DIGE Analysis of Human Tissues

Cecilia Gelfi and Daniele Capitanio

12. DIGE Analysis of Animal Tissues

Alessio Di Luca, Ruth Hamill, Anne Maria Mullen, and Giuliano Elia

13. Rapid 2D DIGE Proteomic Analysis of Mouse Liver

Shotaro Kamata and Isao Ishii

14. Proteomic Analysis of Lung Tissue by DIGE

Jarlath Nally and Simone Schuller

15. Comparative Testis Tissue Proteomics using 2-dye versus 3-dye DIGE Analysis

Ashling Holland

16. DIGE Analysis of Fish Tissues

Joanna Nynca, Mariola Dietrich and Andrzej Ciereszko

Part V: Auxillary DIGE Methods

17. Protein Digestion for DIGE Analysis

Sandra Murphy and Kay Ohlendieck

18. Subcellular Fractionation for DIGE-based Proteomics

Sandra Murphy

19. DIGE Analysis of Immunodepleted Plasma

Paul Dowling and Kay Ohlendieck

Part VI: DIGE Verification Analysis

20. Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays

Andrew Dowd

21. Enzyme Assay Methods to Validate DIGE Proteomics Data

Andrew Dowd

22. Immunoblot Analysis of DIGE-based Proteomics

Martin Landsberger and Heinrich Brinkmeier

23. Immunofluorescence Microscopy for DIGE-based Proteomics

Rustam Mundegar, Margit Zweyer, and Dieter Swandulla

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Product Details
  • ISBN-13: 9781493972678
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: 1st ed. 2018
  • Language: English
  • Returnable: Y
  • Spine Width: 19 mm
  • Weight: 834 gr
  • ISBN-10: 1493972677
  • Publisher Date: 11 Oct 2017
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 318
  • Series Title: Methods in Molecular Biology
  • Sub Title: Methods and Protocols
  • Width: 178 mm

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