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DNA Methylation Protocols

DNA Methylation Protocols


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About the Book

Preface...Table of Contents...Contributing Authors...

Part I Introduction
1. A Summary of the Biological Processes, Disease-Associated Changes and Clinical Applications of DNA MethylationGitte Brinch Andersen and Jörg Tost
2. Considerations for Design and Analysis of DNA Methylation StudiesKarin B. Michels and Alexandra M. Binder
Part II Global DNA Methylation Levels
3. Quantification of Global DNA Methylation Levels by Mass SpectrometryAgustin F. Fernandez, Luis Valledor, Fernando Vallejo, Maria Jesús Cañal, and Mario F. Fraga
4. Antibody Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections and Mammaliam EmbryosNathalie Beaujean, Juliette Salvaing, Nur Annies Abd Hadi, and Sari Pennings
Part III Genome-Wide DNA Methylation Analysis
5. Whole-Genome Bisulfite Sequencing Using the Ovation(R) Ultralow Methyl-Seq ProtocolChristian Daviaud, Victor Renault, Florence Mauger, Jean-François Deleuze, and Jörg Tost 6. Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite SequencingDieter Weichenhan, Qi Wang, Andrew Adey, Stephan Wolf, Jay Shendure, Roland Eils, and Christoph Plass
7. Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing
Fumihito Miura and Takashi Ito
8. Multiplexed Reduced Representation Bisulfite Sequencing with Magnetic Bead Fragment Size SelectionWilliam P. Accomando and Karin B. Michels
9. Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging ApproachJulian R. Peat and Sébastien A. Smallwood

10. Methyl-CpG Binding Domain Sequencing: MBD-seq
Karolina A. Aberg, Robin F. Chan, Linying Xie, Andrey Shabalin, and Edwin J.C.G. van den Oord

11. The HELP-Based AssaysJohn M. Greally
12. Comprehensive Whole DNA Methylome Analysis by Integrating MeDIP-seq and MRE-seqXiaoyun Xing, Bo Zhang, Daofeng Li, and Ting Wang
13. Digital Restriction Enzyme Analysis of Methylation (DREAM)Jaroslav Jelinek, Justin T. Lee, Matteo Cesaroni, Jozef Madzo, Shoudan Liang, Yue Lu, and Jean-Pierre J. Issa
14. Nucleosome Occupancy and Methylome Sequencing (NOMe-seq)Fides D. Lay, Theresa K. Kelly, and Peter A. Jones
15. Bisulfite Sequencing of Chromatin Immunoprecipitated DNA (BisChIP-seq)Clare Stirzaker, Jenny Z. Song, Aaron L. Statham, and Susan J. Clarkdiv>
16. A Guide to Illumina BeadChip Data AnalysisMichael C. Wu and Pei-Fen Kuan
Part IV Highly Multiplexed Target Regions

17. Microdroplet PCR for Highly-Multiplexed Targeted Bisulfite SequencingH. Kiyomi Komori, Sarah A. LaMere, Traver Hart, Steven R. Head, Ali Torkamani, and Daniel R. Salomon
18. Multiplexed DNA Methylation Analysis of Target Regions Using Microfluidics (Fluidigm)Martyna Adamowicz-Brice, Klio Maratou, and Timothy J. Aitman
19. Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock ProbesDinh Diep, Nongluk Plongthonkum, and Kun Zhang
20. Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment SystemJennifer Wendt, Heidi Rosenbaum, Todd Richmond, Jeffrey A. Jeddeloh, and Daniel Burgess

21. Multiplexed and Sensitive DNA Methylation Testing Using Methylation-Sensitive Restriction Enzymes MSRE-qPCR Gabr


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Product Details
  • ISBN-13: 9781493974795
  • Publisher: Humana Press
  • Publisher Imprint: Humana Press
  • Edition: Methods in Molecular Biology
  • Language: English
  • Returnable: Y
  • Spine Width: 38 mm
  • Width: 178 mm
  • ISBN-10: 1493974793
  • Publisher Date: 10 Dec 2017
  • Binding: Hardback
  • Height: 254 mm
  • No of Pages: 704
  • Series Title: Methods in Molecular Biology
  • Weight: 1509 gr


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