Part I. Genetic and Molecular Biological Approaches with Yeast
1. Methods to Study Mitotic Homologous Recombination and Genome Stability
Xiuzhong Zheng, Anastasiya Epstein, and Hannah L. Klein
2. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends
Wenjian Ma, Jim Westmoreland, Wataru Nakai, Anna Malkova, and Michael A. Resnick
3. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis
Sarah Farmer, Wing-Kit Leung, and Hideo Tsubouchi
4. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA
Hannah G. Blitzblau and Andreas Hochwagen
5. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes
Edgar Hartsuiker
6. Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast
Scott Houghtaling, Toyoko Tsukuda, and Mary Ann Osley
7. Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis
Jasvinder A. Singh and G. Valentin Börner
8. Mapping of Crossover Sites Using DNA Microarrays
Stacy Y. Chen and Jennifer C. Fung
9. Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1
Hsiao-Chi Lo and Nancy M. Hollingsworth
10. Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae
Belén Gómez-González, José F. Ruiz, and Andrés Aguilera
11. In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae
Samantha Stuckey, Kuntal Mukherjee, and Francesca Storici
12. Detection of RNA-Templated Double-Strand Break Repair in Yeast
Ying Shen and Francesca Storici
Part II. Genetic and Molecular Biological Approaches with Higher Eukaryotes
13. SNP-Based Mapping of Crossover Recombination in C. elegans
Grace C. Bazan and Kenneth J. Hillers
14. Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana
Jan Drouaud and Christine Mézard
15. Isolation of Meiotic Recombinants from Mouse Sperm
Francesca Cole and Maria Jasin
16. Homologous Recombination Assay for Interstrand Crosslink Repair
Koji Nakanishi, Francesca Cavallo, Erika Brunet, and Maria Jasin
17. Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40
Hiroyuki Kitao, Seiki Hirano, and Minoru Takata
18. Understanding the Immunoglobulin Locus Specificity of Hypermutation
Vera Batrak, Artem Blagodatski, and Jean-Marie Buerstedde
Part III. In Vitro Reconstitution of Homologous Recombination Reactions and Single Molecular Analysis of Recombination Proteins
19. Quality Control of Purified Proteins Involved in Homologous Recombination
Xiao-Ping Zhang and Wolf-Dietrich Heyer
20. Assays for Structure-Selective DNA Endonucleases
William D. Wright, Kirk T. Ehmsen, and Wolf-Dietrich Heyer
21. In vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis
Jie Liu, Jessica Sneeden, and Wolf-Dietrich Heyer
22. An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase
Yasuto Murayama and Hiroshi Iwasaki
23. Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro
Matth
