1. DNA repair: Front and Center and Not Going Away!
Vilhelm A. Bohr
Part I. Identification of Repair Genes and Generation of Repair Mutants
2. Identification of Genes Required for Damage Survival using a Cell Based RNAi Screen against the Drosophila Genome
Dashnamoorthy Ravi and Alexander James Roy Bishop
3. Isolating Genes Involved with Genotoxic Drug Response in the Nematode Caenorhabditis elegans using Genome-wide RNAi Screening
Lone Vedel Schøler, Tine Hørning Møller, Steffen Nørgaard, Lotte Vestergaard and Anders Olsen.
4. Establishment of the DNA Repair-defective Mutants in DT40 Cells
Masamichi Ishiai, Emi Uchida, and Minoru Takata
Part II. Assessing DNA Damage and Repair
5. Electrophoretic Mobility Shift Assays for
Protein-DNA Complexes Involved in DNA Repair
Chun Tsai, PhD, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu
6. The Comet Assay A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair
Günter Speit and Andreas Rothfuss
7. Detection of DNA Damage by Comet Fluorescence in situ Hybridization (Comet-FISH)
W. Schlörmann and M. Glei
8. Quantitative DNA Damage and Repair Measurement with the Yeast Comet Assay
Rui Oliveira and Björn Johansson
9. Analysis of DNA Damage and Repair in Nuclear and Mitochondrial DNA of Animal Cells using Quantitative PCR
Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, Bennett Van Houten
Part III. Repair of Incorrect or Damaged DNA Bases
10. In vitro DNA Mismatch Repair in Human Cells
Liya Gu, Charles M. Ensor, and Guo-Min Li
11. Eukaryotic DNA Mismatch Repair in vitro
Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, Yanbin Zhang
12. Immuno-slot Blot Assay for Detection of UVR-mediated DNA Damage
Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke
13. Quantification of DNA Photoproductions in Mammalian Cell DNAUsing Radioimmunoassay
Thomas R. Berton and David L. Mitchell
14. Measuring the Formation and Repair of UV Damage at the DNA Sequence Level by Ligation-mediated PCR
Ahmad Besaratinia and Gerd P. Pfeifer
15. Construction of Plasmids Containing Site-specific DNA Interstrand Crosslinks for Biochemical and Cell Biological Studies
Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Schärer
16. Replication-coupled DNA Interstrand Crosslink Repair in Xenopus Egg Extracts
Puck Knipscheer, Markus Räschle, Orlando D. Schärer, and Johannes C. Walter
17. In Vitro Base Excision Repair using Mammalian Cell Extracts
Jason L. Parsons and Grigory L. Dianov,
18. Using Arabidopsis Cell Extracts to Monitor Repair of DNA Base Damage in vitro
Dolores Córdoba-Cañero, Teresa Roldán-Arjona and Rafael R. Ariza
19. In vitro monitoring of Base Excision Repair in Saccharomyces cerevisiae
Simone Moertl and Fred Ahne
20. Mitochondrial Base Excision Repair Assays
Ricardo Gredilla and Tinna Stevnsner
Part IV. DNA Strand Breakage and Repair
21. O-Hydroxylamine-Coupled Alkaline Gel Electrophoresis Assay for the Detection and Measurement of DNA Single-Strand Breaks
April M. Luke and Jun Nakamura
22. DNA Double Stand Break Damage and Repair Assessed by Pulse-field Gel
Electrophoresis
Helen E. Bryant
23. Quantitative Amplification of Single Stranded DNA (QAOS)
Eva-Maria Holstein and David Lydall
24. Simple, Non-radioactive Measurement of Single-stranded DNA at Telomeric, Sub-telomeri
